Document Detail


Biological nanofactories target and activate epithelial cell surfaces for modulating bacterial quorum sensing and interspecies signaling.
MedLine Citation:
PMID:  21028779     Owner:  NLM     Status:  In-Process    
Abstract/OtherAbstract:
In order to control the behavior of bacteria present at the surface of human epithelial cells, we have created a biological "nanofactory" construct that "coats" the epithelial cells and "activates" the surface to produce the bacterial quorum sensing signaling molecule, autoinducer-2 (AI-2). Specifically, we demonstrate directed modulation of signaling among Escherichia coli cells grown over the surface of human epithelial (Caco-2) cells through site-directed attachment of biological nanofactories. These "factories" comprise a fusion protein expressed and purified from E. coli containing two AI-2 bacterial synthases (Pfs and LuxS), a protein G IgG binding domain, and affinity ligands for purification. The final factory is fabricated ex vivo by incubating with an anti-CD26 antibody that binds the fusion protein and specifically targets the CD26 dipeptidyl peptidase found on the outer surface of Caco-2 cells. This is the first report of the intentional "in vitro" synthesis of bacterial autoinducers at the surface of epithelial cells for the redirection of quorum sensing behaviors of bacteria. We envision tools such as this will be useful for interrogating, interpreting, and disrupting signaling events associated with the microbiome localized in human intestine and other environments.
Authors:
Colin G Hebert; Apoorv Gupta; Rohan Fernandes; Chen-Yu Tsao; James J Valdes; William E Bentley
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2010-10-28
Journal Detail:
Title:  ACS nano     Volume:  4     ISSN:  1936-086X     ISO Abbreviation:  ACS Nano     Publication Date:  2010 Nov 
Date Detail:
Created Date:  2010-11-24     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101313589     Medline TA:  ACS Nano     Country:  United States    
Other Details:
Languages:  eng     Pagination:  6923-31     Citation Subset:  IM    
Affiliation:
Center for Biosystems Research, University of Maryland Biotechnology Institute, 5115 Plant Science Building, College Park, Maryland 20742, United States.
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