| Biologic properties of mesenchymal stem cells derived from bone marrow and adipose tissue. | |
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MedLine Citation:
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PMID: 16795045 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The biologic characteristics of mesenchymal stem cells (MSCs) isolated from two distinct tissues, bone marrow and adipose tissue were evaluated in these studies. MSCs derived from human and non-human primate (rhesus monkey) tissue sources were compared. The data indicate that MSCs isolated from rhesus bone marrow (rBMSCs) and human adipose tissue (hASCs) had more similar biologic properties than MSCs of rhesus adipose tissue (rASCs) and human bone marrow MSCs (hBMSCs). Analyses of in vitro growth kinetics revealed shorter doubling time for rBMSCs and hASCs. rBMSCs and hASCs underwent significantly more population doublings than the other MSCs. MSCs from all sources showed a marked decrease in telomerase activity over extended culture; however, they maintained their mean telomere length. All of the MSCs expressed embryonic stem cell markers, Oct-4, Rex-1, and Sox-2 for at least 10 passages. Early populations of MSCs types showed similar multilineage differentiation capability. However, only the rBMSCs and hASCs retain greater differentiation efficiency at higher passages. Overall in vitro characterization of MSCs from these two species and tissue sources revealed a high level of common biologic properties. However, the results demonstrate clear biologic distinctions, as well. |
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Authors:
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Reza Izadpanah; Cynthia Trygg; Bindiya Patel; Christopher Kriedt; Jason Dufour; Jeffery M Gimble; Bruce A Bunnell |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of cellular biochemistry Volume: 99 ISSN: 0730-2312 ISO Abbreviation: J. Cell. Biochem. Publication Date: 2006 Dec |
Date Detail:
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Created Date: 2006-11-02 Completed Date: 2007-05-31 Revised Date: 2007-12-03 |
Medline Journal Info:
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Nlm Unique ID: 8205768 Medline TA: J Cell Biochem Country: United States |
Other Details:
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Languages: eng Pagination: 1285-97 Citation Subset: IM |
Copyright Information:
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2006 Wiley-Liss, Inc. |
Affiliation:
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Division of Gene Therapy, Tulane National Primate Center, Tulane University Health Sciences Center, Covington, Louisiana 70433, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adipose Tissue
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cytology* Animals Biological Markers / metabolism Bone Marrow Cells / cytology, physiology* Cell Differentiation / physiology* Cell Lineage Cells, Cultured Chondrogenesis / physiology Humans Macaca mulatta Mesenchymal Stem Cell Transplantation Mesenchymal Stem Cells / cytology, physiology* Osteogenesis / physiology Telomerase / metabolism Telomere / metabolism Transcription Factors / metabolism |
| Grant Support | |
ID/Acronym/Agency:
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RR00164/RR/NCRR NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Biological Markers; 0/Transcription Factors; EC 2.7.7.49/Telomerase |
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