| Biologic and physical characteristics of the non-peptidic, non-digitalis-like natriuretic hormone. | |
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MedLine Citation:
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PMID: 8264153 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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At least three independent groups of natriuretic hormones have been isolated over the past ten years. Two, atrial natriuretic factor (ANF) and brain natriuretic peptide (BNP), are proteins and the third is made up of digitalis-like substances (DLS). The present report concerns the isolation, substantial purification and biologic actions of an entirely different natriuretic hormone (NH) which appears to be steroidal in nature and an isomer of cortisone. The source of NH was uremic urine. Purification involved successive chromatographic steps including gel filtration and multiple HPLC runs through C-18 resins. A translucent crystal ultimately was obtained. The product was examined using mass spectroscopy with trimethylsilyl derivatization. Only one compound was identifiable. The characteristics of the molecule include: a molecular weight, 360.4; a molecular formula, C21H28O5; a steroidal nucleus; UV absorption at 220 and 290 nm; and intrinsic fluorescence. The onset of action occurs within minutes both in the rat and, as previously shown, in several in vitro systems including the frog skin, toad bladder, fibroblasts and renal tubular epithelial cells grown in culture and isolated perfused cortical collecting tubules. In contrast to DLS, NH has been previously shown not to cross react with digoxin antibodies. Moreover, when given to intact rats, it produces a profound natriuresis but little or no kaliuresis. In contrast to ANF and BNP the compound is active orally as well as intravenously. It is clearly different from cortisone, based both on its biologic and mass spectroscopic characteristics. |
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Authors:
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N S Bricker; L Zea; M Shapiro; E Sanclemente; S Shankel |
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Publication Detail:
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Type: Comparative Study; Journal Article |
Journal Detail:
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Title: Kidney international Volume: 44 ISSN: 0085-2538 ISO Abbreviation: Kidney Int. Publication Date: 1993 Nov |
Date Detail:
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Created Date: 1994-01-26 Completed Date: 1994-01-26 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0323470 Medline TA: Kidney Int Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 937-47 Citation Subset: IM |
Affiliation:
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Center for Kidney Research, Loma Linda University Medical Center, School of Medicine, California. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Administration, Oral Animals Chromatography, Gel Chromatography, High Pressure Liquid Female Humans Natriuresis / drug effects Natriuretic Agents / isolation & purification, pharmacology, physiology* Rats Rats, Sprague-Dawley Uremia / urine |
| Chemical | |
Reg. No./Substance:
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0/Natriuretic Agents |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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