Document Detail

Biolabeling with 2,4-dichlorophenoxyacetic acid derivatives: the 2,4-D tag.
MedLine Citation:
PMID:  19873999     Owner:  NLM     Status:  MEDLINE    
Many bioanalytic and diagnostic procedures rely on labels with which the molecule of interest can be tracked in or discriminated from accompanying like substances. Herein, we describe a new labeling and detection system based on derivatives of 2,4-dichlorophenoxyacetic acid (2,4-D) and anti-2,4-D antibodies. The 2,4-D system is highly sensitive with a K(D) of 7 x 10(-11) M for the hapten-antibody pair, can be used on a large variety of biomolecules such as proteins, peptides, carbohydrates, and nucleic acids, is not hampered by endogenous backgrounds because 2,4-D is a xenobiotic, and is robust because 2,4-D is a very stable compound that withstands the conditions of most reactions usually performed on biomolecules. With this unique blend of properties, the 2,4-D system compares favorably with its rivals digoxigenin (DIG)/anti-DIG and biotin/(strept)avidin and provides an interesting and powerful tool in biomolecular labeling.
Steffen Bade; Niels R?ckendorf; Milan Franek; Hans H Gorris; Buko Lindner; Verena Olivier; Klaus-J?rgen Schaper; Andreas Frey
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Analytical chemistry     Volume:  81     ISSN:  1520-6882     ISO Abbreviation:  Anal. Chem.     Publication Date:  2009 Dec 
Date Detail:
Created Date:  2009-12-01     Completed Date:  2010-02-23     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0370536     Medline TA:  Anal Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  9695-702     Citation Subset:  IM    
Division of Mucosal Immunology, Department of Pneumology, Research Center Borstel, Parkallee 22, 23845 Borstel, Germany.
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MeSH Terms
2,4-Dichlorophenoxyacetic Acid / analogs & derivatives*,  analysis,  immunology,  metabolism*
Amines / metabolism
Antibodies, Monoclonal / immunology
DNA / metabolism
Deoxyuracil Nucleotides / chemistry,  metabolism
Immunoassay / methods*
Limit of Detection
Polyethylene Glycols / metabolism
Proteins / chemistry,  metabolism
Staining and Labeling / methods*
Reg. No./Substance:
0/Amines; 0/Antibodies, Monoclonal; 0/Deoxyuracil Nucleotides; 0/Polyethylene Glycols; 0/Proteins; 1173-82-6/deoxyuridine triphosphate; 9007-49-2/DNA; 94-75-7/2,4-Dichlorophenoxyacetic Acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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