Document Detail


Bioimaging of Peroxynitrite in MCF-7 Cells by a New Fluorescent Probe Rhodamine B Phenyl Hydrazide.
MedLine Citation:
PMID:  23479207     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Peroxynitrite is a potent oxidizing and nitrating agent which has detrimental effects on cells by altering the structure and function of biomolecules present within. A fluorescent probe rhodamine B phenyl hydrazide (RBPH) has been proposed for peroxynitrite (ONOO-) imaging in MCF-7 cells based on its oxidation property, which converts RBPH to pink colored and highly fluorescent rhodamine B. The fluorescence emission intensity of the rhodamine B produced in the above process is linearly related to the concentration of peroxynitrite. The method obeys Beer's law in the concentration range 2-20 nM and the detection limit has been found to be 1.4 nM. The possible reaction mechanism of peroxynitrite with RBPH to produce rhodamine B has been discussed with spectroscopic evidence. The Probe is selective to the peroxynitrite in the pH range 6-8 which is near physiological pH. Fluorescence microscopic studies suggest that the probe is cell permeable and hence peroxynitrite was imaged in MCF-7 cells.
Authors:
Gopi Ambikapathi; Suresh Kumar Kempahanumakkagari; Babu Ramappa Lamani; Devaraju Kuramkote Shivanna; Harish Bodagur Maregowda; Anushree Gupta; Pandurangappa Malingappa
Related Documents :
23876447 - Cell adhesion on zein films under shear stress field.
3313807 - Multicentric giant lymph node hyperplasia, plasma cell type, with monoclonal gammopathy.
23871837 - The evolution of alternative biofilms in an opportunistic fungal pathogen: an explanati...
24460377 - Neuroprotective action of deer bone extract against glutamate or aβ1-42-induced oxidati...
19571257 - Role of isoprenylation in simvastatin-induced inhibition of ovarian theca-interstitial ...
16137497 - An immunohistochemical analysis of human merkel cell density in gingival epithelium fro...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-3-12
Journal Detail:
Title:  Journal of fluorescence     Volume:  -     ISSN:  1573-4994     ISO Abbreviation:  J Fluoresc     Publication Date:  2013 Mar 
Date Detail:
Created Date:  2013-3-12     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9201341     Medline TA:  J Fluoresc     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
Department of Microbiology & Biotechnology, Bangalore University, Jnanabharathi Campus, Bangalore, 560 056, India.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Indole-based Fluorescent Sensors for Selective Detection of Hg2+
Next Document:  Ki-67 evaluation at the hottest spot predicts clinical outcome of patients with hormone receptor-pos...