| Biohydrogenation of Linolenic Acid to Stearic Acid by the Rumen Microbial Population Yields Multiple Intermediate Conjugated Diene Isomers. | |
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MedLine Citation:
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PMID: 21653571 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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The current literature suggests that linolenic acid biohydrogenation converts to stearic acid without the formation of CLA. However, a multitude of CLA were identified in the rumen that are generally attributed to linoleic acid biohydrogenation. This study used a stable isotope tracer to investigate the biohydrogenation intermediates of (13)C-linolenic acid, including CLA. A continuous culture fermenter was used to maintain a mixed microbial population obtained from the rumen of cattle at pH 6.5 for 6 d. The mixed fermenter contents were then transferred to batch cultures containing either (13)C-labeled or unlabeled linolenic acid, which were run in triplicate for 0, 3, 24, and 48 h. The (13)C enrichment was determined by GC-MS. After 48 h of incubation, 8 CLA isomers were significantly enriched, suggesting that these CLA isomers originated directly from linolenic acid. The cis-10 cis-12 CLA isomer exhibited the highest enrichment (21.7%), followed by cis-9 cis-11 and trans-8 trans-10 CLA. The enrichment of these 2 CLA isomers ranged from 20.1 to 21.1% and the remaining 5 CLA including cis-9 trans-11 CLA were <15.0%. A multitude of nonconjugated and partially conjugated 18:2 and 18:3 isomers was enriched during the 48 h of incubation. The results of this study confirm that mixed ruminal microbes are capable of the formation of several CLA and 18:3 isomers from linolenic acid, indicating that linolenic acid biohydrogenation pathways are more complex than previously reported. |
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Authors:
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Yong-Jae Lee; Thomas C Jenkins |
Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-6-8 |
Journal Detail:
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Title: The Journal of nutrition Volume: - ISSN: 1541-6100 ISO Abbreviation: - Publication Date: 2011 Jun |
Date Detail:
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Created Date: 2011-6-9 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0404243 Medline TA: J Nutr Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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Department of Animal and Veterinary Sciences, Clemson University, Clemson, SC 29634. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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