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Biogenesis of mitochondria. 13. The isolation of mitochondrial structures from anaerobically grown Saccharomyces cerevisiae.
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MedLine Citation:
PMID:  4248186     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Morphologically intact structures have been isolated from anaerobically grown yeast cells which have many of the properties of yeast mitochondria. The structures are about 0.5 micro in diameter and contain malate dehydrogenase, succinate dehydrogenase, oligomycin-sensitive ATPase, and DNA of buoyant density 1.683 g/cc, characteristic of yeast mitochondria. The morphology of the structures is critically dependent on their lipid composition. When isolated from cells grown anaerobically in the presence of supplements of unsaturated fatty acid and ergosterol, their unsaturated fatty acid content is similar to that of mitochondria from aerobically grown cells. These lipid-complete structures consist pre-dominantly of double-membrane vesicles enclosing a dense matrix which contains a folded inner membrane system bordering electron-transparent regions which are somewhat different from the cristae of functional mitochondria. In contrast, the structures from cells grown without lipid supplements are much simpler in morphology; they have a dense granular matrix surrounded by a double membrane but have no obvious folded inner membrane system within the matrix. The lipid-depleted structures are very fragile and are only isolated in intact form from protoplasts that have been prefixed with glutaraldehyde
Authors:
K Watson; J M Haslam; A W Linnane
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Journal of cell biology     Volume:  46     ISSN:  0021-9525     ISO Abbreviation:  J. Cell Biol.     Publication Date:  1970 Jul 
Date Detail:
Created Date:  1970-11-06     Completed Date:  1970-11-06     Revised Date:  2010-06-22    
Medline Journal Info:
Nlm Unique ID:  0375356     Medline TA:  J Cell Biol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  88-96     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Adenosine Triphosphatases / metabolism
Centrifugation, Density Gradient
DNA / metabolism
Fats, Unsaturated / metabolism
Malate Dehydrogenase / metabolism
Microscopy, Electron
Mitochondria* / enzymology
Saccharomyces / cytology*
Succinate Dehydrogenase / metabolism
Chemical
Reg. No./Substance:
0/Fats, Unsaturated; 9007-49-2/DNA; EC 1.1.1.37/Malate Dehydrogenase; EC 1.3.99.1/Succinate Dehydrogenase; EC 3.6.1.-/Adenosine Triphosphatases
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Journal Information
Journal ID (nlm-ta): J Cell Biol
ISSN: 0021-9525
ISSN: 1540-8140
Publisher: The Rockefeller University Press
Article Information
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Copyright © 1970 by The Rockefeller University Press
Received Day: 10 Month: 12 Year: 1969
Revision Received Day: 29 Month: 1 Year: 1970
Print publication date: Day: 1 Month: 7 Year: 1970
Volume: 46 Issue: 1
First Page: 88 Last Page: 96
ID: 2108061
PubMed Id: 4248186

BIOGENESIS OF MITOCHONDRIA : XIII. The Isolation of Mitochondrial Structures from Anaerobically Grown Saccharomyces cerevisiae
K. Watson
J. M. Haslam
Anthony W. Linnane
From the Biochemistry Department, Monash University, Clayton, Victoria, 3168, Australia


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