| Biogenesis and cell cycle relationship of poly(A)- actin mRNA in mouse ascites cells. | |
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MedLine Citation:
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PMID: 2866105 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A variety of rapidly growing mammalian cells contain a substantial portion of their actin mRNA in a poly(A)- form. We have used DNA-driven hybridization of a cloned actin cDNA-containing plasmid with pulse-labeled RNA from mouse S-180 ascites cells to examine newly synthesized actin mRNA. Our results indicate that the same proportion of newly synthesized and steady-state actin mRNA (approx. 40%) exists in a poly(A)- deficient form. This suggests that the poly(A)- form arises by some process other than slow cytoplasmic de-adenylation of a poly(A)+ precursor. We have also examined cell cycle-enriched populations of S-180 ascites cells for the presence of poly(A)- actin mRNA. Results from these experiments indicate that cells in G1 phase of the cell cycle contain predominantly poly(A)+ actin mRNA, while the poly(A)- form is restricted to late-S and post-S phase cells. |
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Authors:
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T E Geoghegan; L McCoy |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Experimental cell research Volume: 162 ISSN: 0014-4827 ISO Abbreviation: Exp. Cell Res. Publication Date: 1986 Jan |
Date Detail:
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Created Date: 1986-01-14 Completed Date: 1986-01-14 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0373226 Medline TA: Exp Cell Res Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 175-82 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Actins
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genetics* Animals Ascitic Fluid / pathology Cells, Cultured DNA Interphase* Mice Nucleic Acid Hybridization Protein Biosynthesis RNA, Messenger / analysis, genetics, metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Actins; 0/RNA, Messenger; 9007-49-2/DNA |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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