Document Detail

Biogenesis and cell cycle relationship of poly(A)- actin mRNA in mouse ascites cells.
MedLine Citation:
PMID:  2866105     Owner:  NLM     Status:  MEDLINE    
A variety of rapidly growing mammalian cells contain a substantial portion of their actin mRNA in a poly(A)- form. We have used DNA-driven hybridization of a cloned actin cDNA-containing plasmid with pulse-labeled RNA from mouse S-180 ascites cells to examine newly synthesized actin mRNA. Our results indicate that the same proportion of newly synthesized and steady-state actin mRNA (approx. 40%) exists in a poly(A)- deficient form. This suggests that the poly(A)- form arises by some process other than slow cytoplasmic de-adenylation of a poly(A)+ precursor. We have also examined cell cycle-enriched populations of S-180 ascites cells for the presence of poly(A)- actin mRNA. Results from these experiments indicate that cells in G1 phase of the cell cycle contain predominantly poly(A)+ actin mRNA, while the poly(A)- form is restricted to late-S and post-S phase cells.
T E Geoghegan; L McCoy
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Experimental cell research     Volume:  162     ISSN:  0014-4827     ISO Abbreviation:  Exp. Cell Res.     Publication Date:  1986 Jan 
Date Detail:
Created Date:  1986-01-14     Completed Date:  1986-01-14     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0373226     Medline TA:  Exp Cell Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  175-82     Citation Subset:  IM    
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MeSH Terms
Actins / genetics*
Ascitic Fluid / pathology
Cells, Cultured
Nucleic Acid Hybridization
Protein Biosynthesis
RNA, Messenger / analysis,  genetics,  metabolism*
Reg. No./Substance:
0/Actins; 0/RNA, Messenger; 9007-49-2/DNA

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