Document Detail


Biogenesis of apolipoprotein A-V and its impact on VLDL triglyceride secretion.
MedLine Citation:
PMID:  21115968     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Apolipoprotein A-V (apoA-V) is a potent regulator of intravascular triglyceride (TG) metabolism, yet its plasma concentration is very low compared with that of other apolipoproteins. To examine the basis for its low plasma concentration, the secretion efficiency of apoA-V was measured in stably transfected McA-RH7777 rat hepatoma cells. Pulse-chase experiments revealed that only ∼20% of newly synthesized apoA-V is secreted into culture medium within 3 h postsynthesis and that ∼65% undergoes presecretory turnover; similar results were obtained with transfected nonhepatic Chinese hamster ovary cells. ApoA-V secreted by McA-RH7777 cells was not associated with cell surface heparin-competable binding sites. When stably transfected McA-RH7777 cells were treated with oleic acid, the resulting increase in TG synthesis caused a reduction in apoA-V secretion, a reciprocal increase in cell-associated apoA-V, and movement of apoA-V onto cytosolic lipid droplets. In a stably transfected doxycycline-inducible McA-RH7777 cell line, apoA-V expression inhibited TG secretion by ∼50%, increased cellular TG, and reduced Z-average VLDL(1) particle diameter from 81 to 67 nm; however, no impact on apoB secretion was observed. These data demonstrate that apoA-V inefficiently traffics within the secretory pathway, that its intracellular itinerary can be regulated by changes in cellular TG accumulation, and that apoA-V synthesis can modulate VLDL TG mobilization and secretion.
Authors:
Anna M Blade; Melissa A Fabritius; Li Hou; Richard B Weinberg; Gregory S Shelness
Related Documents :
10942588 - Electric cell-substrate impedance sensing (ecis) as a noninvasive means to monitor the ...
19662408 - Nuclisome: a novel concept for radionuclide therapy using targeting liposomes.
17819308 - Molecular weight determinations.
3782308 - Osteoclasts and osteoblasts migrate in opposite directions in response to a constant el...
6252118 - Two-hit kinetics of focus formation in cells transfected with rous sarcoma provirus.
7160308 - Nerve growth factor requirement of postnatal rat adrenal medullary cells in vitro for s...
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2010-11-26
Journal Detail:
Title:  Journal of lipid research     Volume:  52     ISSN:  0022-2275     ISO Abbreviation:  J. Lipid Res.     Publication Date:  2011 Feb 
Date Detail:
Created Date:  2011-01-17     Completed Date:  2011-05-05     Revised Date:  2013-07-03    
Medline Journal Info:
Nlm Unique ID:  0376606     Medline TA:  J Lipid Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  237-44     Citation Subset:  IM    
Affiliation:
Department of Pathology, Wake Forest University School of Medicine, Winston-Salem, NC 27157, USA.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Apolipoproteins / biosynthesis*,  metabolism
CHO Cells
Cricetinae
Cricetulus
Lipoproteins, VLDL / secretion*
Liver Neoplasms, Experimental / metabolism
Oleic Acid / metabolism
Rats
Transfection
Triglycerides / secretion*
Grant Support
ID/Acronym/Agency:
HL-30897/HL/NHLBI NIH HHS; HL-49373/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/Apoa5 protein, rat; 0/Apolipoproteins; 0/Lipoproteins, VLDL; 0/Triglycerides; 0/very low density lipoprotein triglyceride; 112-80-1/Oleic Acid
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  A novel ELISA for measuring CD36 protein in human adipose tissue.
Next Document:  Mapping the epistatic network underlying murine reproductive fatpad variation.