| Biofilms of non-Candida albicans Candida species: quantification, structure and matrix composition. | |
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MedLine Citation:
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PMID: 19888800 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Most cases of candidiasis have been attributed to C. albicans, but recently, non- Candida albicans Candida (NCAC) species have been identified as common pathogens. The ability of Candida species to form biofilms has important clinical repercussions due to their increased resistance to antifungal therapy and the ability of yeast cells within the biofilms to withstand host immune defenses. Given this clinical importance of the biofilm growth form, the aim of this study was to characterize biofilms produced by three NCAC species, namely C. parapsilosis, C. tropicalis and C. glabrata. The biofilm forming ability of clinical isolates of C. parapsilosis, C. tropicalis and C. glabrata recovered from different sources, was evaluated by crystal violet staining. The structure and morphological characteristics of the biofilms were also assessed by scanning electron microscopy and the biofilm matrix composition analyzed for protein and carbohydrate content. All NCAC species were able to form biofilms although these were less extensive for C. glabrata compared with C. parapsilosis and C. tropicalis. It was evident that C. parapsilosis biofilm production was highly strain dependent, a feature not evident with C. glabrata and C. tropicalis. Scanning electron microscopy revealed structural differences for biofilms with respect to cell morphology and spatial arrangement. Candida parapsilosis biofilm matrices had large amounts of carbohydrate with less protein. Conversely, matrices extracted from C. tropicalis biofilms had low amounts of carbohydrate and protein. Interestingly, C. glabrata biofilm matrix was high in both protein and carbohydrate content. The present work demonstrates that biofilm forming ability, structure and matrix composition are highly species dependent with additional strain variability occurring with C. parapsilosis. |
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Authors:
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S?nia Silva; Mariana Henriques; Ant?nio Martins; Ros?rio Oliveira; David Williams; Joana Azeredo |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Medical mycology : official publication of the International Society for Human and Animal Mycology Volume: 47 ISSN: 1460-2709 ISO Abbreviation: Med. Mycol. Publication Date: 2009 Nov |
Date Detail:
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Created Date: 2009-11-05 Completed Date: 2010-02-12 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9815835 Medline TA: Med Mycol Country: England |
Other Details:
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Languages: eng Pagination: 681-9 Citation Subset: IM |
Affiliation:
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Institute for Biotechnology and Bioengineering, Universidade do Minho, Campus de Gualtar 4710-057, Braga, Portugal. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Biofilms
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growth & development* Candida / chemistry*, cytology, growth & development, isolation & purification Candidiasis / microbiology* Carbohydrates / analysis* Female Fungal Proteins / analysis* Humans Mouth / microbiology Urinary Tract / microbiology Vagina / microbiology |
| Chemical | |
Reg. No./Substance:
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0/Carbohydrates; 0/Fungal Proteins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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