Document Detail

Biofilm in milking equipment on a dairy farm as a potential source of bulk tank milk contamination with Listeria monocytogenes.
MedLine Citation:
PMID:  20494189     Owner:  NLM     Status:  MEDLINE    
The objective of this study was to assess the presence of a Listeria monocytogenes-containing biofilm in milking equipment as a potential source of bulk tank milk contamination on a dairy farm where milk contamination had been previously documented. Samples were collected from milking equipment and milking parlor premises on 4 occasions and analyzed for the presence of L. monocytogenes. Pulsed-field gel electrophoresis (PFGE) typing was conducted on L. monocytogenes isolates from the milking equipment, parlor and storage room floors, bulk tank milk, and in-line milk filters. Pieces from milk meters and rubber liners were obtained to visually assess the presence of a biofilm using scanning electron microscopy. A total of 6 (15%), 4 (25%), and 1 (6%) samples were culture-positive for L. monocytogenes in the first, second, and third sample collection, respectively. Two samples were L. monocytogenes hly PCR-positive but were culture-negative in the fourth sample collection. Combined AscI and ApaI restriction analysis yielded 6 PFGE types for 15 L. monocytogenes isolates obtained from milking equipment, parlor, bulk tank milk, and milk filters. A predominant and persistent PFGE type (PFGE type T) was observed among these L. monocytogenes isolates (9/15 isolates). Scanning electron microscopy of samples from the bottom cover of 2 milk meters showed the presence of individual and clusters of bacteria, mainly associated with surface scratches. The presence of a bacterial biofilm was observed on the bottom covers of the 2 milk meters. Prevention of the establishment of biofilms in milking equipment is a crucial step in fulfilling the requirement of safe, high-quality milk.
A A Latorre; J S Van Kessel; J S Karns; M J Zurakowski; A K Pradhan; K J Boor; B M Jayarao; B A Houser; C S Daugherty; Y H Schukken
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Journal of dairy science     Volume:  93     ISSN:  1525-3198     ISO Abbreviation:  J. Dairy Sci.     Publication Date:  2010 Jun 
Date Detail:
Created Date:  2010-05-24     Completed Date:  2010-09-03     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  2985126R     Medline TA:  J Dairy Sci     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2792-802     Citation Subset:  IM    
Copyright Information:
2010 American Dairy Science Association. Published by Elsevier Inc. All rights reserved.
Department of Population Medicine and Diagnostic Sciences, Cornell University, Ithaca, NY 14853, USA.
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MeSH Terms
Cattle / microbiology
Dairying* / instrumentation
Electrophoresis, Gel, Pulsed-Field
Food Contamination*
Food Handling
Listeria monocytogenes* / isolation & purification
Microscopy, Electron, Scanning
Milk / microbiology*
Polymerase Chain Reaction

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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