| Biofilm in milking equipment on a dairy farm as a potential source of bulk tank milk contamination with Listeria monocytogenes. | |
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MedLine Citation:
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PMID: 20494189 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The objective of this study was to assess the presence of a Listeria monocytogenes-containing biofilm in milking equipment as a potential source of bulk tank milk contamination on a dairy farm where milk contamination had been previously documented. Samples were collected from milking equipment and milking parlor premises on 4 occasions and analyzed for the presence of L. monocytogenes. Pulsed-field gel electrophoresis (PFGE) typing was conducted on L. monocytogenes isolates from the milking equipment, parlor and storage room floors, bulk tank milk, and in-line milk filters. Pieces from milk meters and rubber liners were obtained to visually assess the presence of a biofilm using scanning electron microscopy. A total of 6 (15%), 4 (25%), and 1 (6%) samples were culture-positive for L. monocytogenes in the first, second, and third sample collection, respectively. Two samples were L. monocytogenes hly PCR-positive but were culture-negative in the fourth sample collection. Combined AscI and ApaI restriction analysis yielded 6 PFGE types for 15 L. monocytogenes isolates obtained from milking equipment, parlor, bulk tank milk, and milk filters. A predominant and persistent PFGE type (PFGE type T) was observed among these L. monocytogenes isolates (9/15 isolates). Scanning electron microscopy of samples from the bottom cover of 2 milk meters showed the presence of individual and clusters of bacteria, mainly associated with surface scratches. The presence of a bacterial biofilm was observed on the bottom covers of the 2 milk meters. Prevention of the establishment of biofilms in milking equipment is a crucial step in fulfilling the requirement of safe, high-quality milk. |
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Authors:
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A A Latorre; J S Van Kessel; J S Karns; M J Zurakowski; A K Pradhan; K J Boor; B M Jayarao; B A Houser; C S Daugherty; Y H Schukken |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, Non-P.H.S. |
Journal Detail:
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Title: Journal of dairy science Volume: 93 ISSN: 1525-3198 ISO Abbreviation: J. Dairy Sci. Publication Date: 2010 Jun |
Date Detail:
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Created Date: 2010-05-24 Completed Date: 2010-09-03 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 2985126R Medline TA: J Dairy Sci Country: United States |
Other Details:
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Languages: eng Pagination: 2792-802 Citation Subset: IM |
Copyright Information:
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2010 American Dairy Science Association. Published by Elsevier Inc. All rights reserved. |
Affiliation:
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Department of Population Medicine and Diagnostic Sciences, Cornell University, Ithaca, NY 14853, USA. aal39@cornell.edu |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Biofilms* Cattle / microbiology Dairying* / instrumentation Electrophoresis, Gel, Pulsed-Field Food Contamination* Food Handling Listeria monocytogenes* / isolation & purification Microscopy, Electron, Scanning Milk / microbiology* Polymerase Chain Reaction |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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