Document Detail


Biofilm formation studies in microtiter plate format.
MedLine Citation:
PMID:  22328388     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Although Candida biofilms have been clearly identified as playing an increasingly important role in human disease, their biology and the reason for their poor susceptibility to antifungal agents remain largely unknown. Over recent years, various models have been developed in order to better characterize Candida biofilms. Here, we describe a number of rapid, inexpensive microtiter-format techniques and strategies which can be used for large-scale screening procedures aimed at identifying genes involved in Candida biofilm formation and/or potential antifungal agents with activity against pathogen cells growing under these conditions. The procedures could also be easily adapted for studying biofilm structures with a range of microscopy techniques.
Authors:
Marta Riera; Emilia Moreno-Ruiz; Sophie Goyard; Christophe d'Enfert; Guilhem Janbon
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Methods in molecular biology (Clifton, N.J.)     Volume:  845     ISSN:  1940-6029     ISO Abbreviation:  Methods Mol. Biol.     Publication Date:  2012  
Date Detail:
Created Date:  2012-02-13     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9214969     Medline TA:  Methods Mol Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  369-77     Citation Subset:  IM    
Affiliation:
Centre for Research in Agricultural Genomics (CRAG), Campus UAB Bellaterra, Cerdanyola del Valles, Barcelona, Spain.
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