Document Detail


Biofilm development and cell death in the marine bacterium Pseudoalteromonas tunicata.
MedLine Citation:
PMID:  15184116     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The newly described green-pigmented bacterium Pseudoalteromonas tunicata (D2) produces target-specific inhibitory compounds against bacteria, algae, fungi, and invertebrate larvae and is frequently found in association with living surfaces in the marine environment. As part of our studies on the ecology of P. tunicata and its interaction with marine surfaces, we examined the ability of P. tunicata to form biofilms under continuous culture conditions within the laboratory. P. tunicata biofilms exhibited a characteristic architecture consisting of differentiated microcolonies surrounded by water channels. Remarkably, we observed a repeatable pattern of cell death during biofilm development of P. tunicata, similar to that recently reported for biofilms of Pseudomonas aeruginosa (J. S. Webb et al., J. Bacteriol. 185:4585-4595, 2003). Killing and lysis occurred inside microcolonies, apparently resulting in the formation of voids within these structures. A subpopulation of viable cells was always observed within the regions of killing in the biofilm. Moreover, extensive killing in mature biofilms appeared to result in detachment of the biofilm from the substratum. A novel 190-kDa autotoxic protein produced by P. tunicata, designated AlpP, was found to be involved in this biofilm killing and detachment. A Delta alpP mutant derivative of P. tunicata was generated, and this mutant did not show cell death during biofilm development. We propose that AlpP-mediated cell death plays an important role in the multicellular biofilm development of P. tunicata and subsequent dispersal of surviving cells within the marine environment.
Authors:
Anne Mai-Prochnow; Flavia Evans; Doralyn Dalisay-Saludes; Sacha Stelzer; Suhelen Egan; Sally James; Jeremy S Webb; Staffan Kjelleberg
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Applied and environmental microbiology     Volume:  70     ISSN:  0099-2240     ISO Abbreviation:  Appl. Environ. Microbiol.     Publication Date:  2004 Jun 
Date Detail:
Created Date:  2004-06-08     Completed Date:  2004-08-10     Revised Date:  2013-06-09    
Medline Journal Info:
Nlm Unique ID:  7605801     Medline TA:  Appl Environ Microbiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  3232-8     Citation Subset:  IM    
Affiliation:
School of Biotechnology and Biomolecular Sciences and Centre for Marine Biofouling and Bio-Innovation, Biological Sciences Building, University of New South Wales, Sydney, NSW 2052, Australia.
Data Bank Information
Bank Name/Acc. No.:
GENBANK/AY295768
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MeSH Terms
Descriptor/Qualifier:
Bacterial Proteins / genetics,  metabolism
Biofilms / growth & development*
Culture Media
Gene Expression Regulation, Bacterial*
Microscopy, Electron
Molecular Sequence Data
Mutagenesis, Site-Directed
Pseudoalteromonas / genetics,  growth & development*
Seawater / microbiology*
Sequence Analysis, DNA
Chemical
Reg. No./Substance:
0/Bacterial Proteins; 0/Culture Media
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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