Document Detail


Biofilm formation within the interface of bovine root dentin treated with conjugated chitosan and sealer containing chitosan nanoparticles.
MedLine Citation:
PMID:  23321239     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
INTRODUCTION: The purpose of this study was to assess biofilm formation within sealer-dentin interfaces of root segments filled with gutta-percha and sealer incorporated with chitosan (CS) nanoparticles with and without canal surface treatment with different formulations of CS.
METHODS: Standardized canals of 4-mm bovine root segments (N = 35) were filled with gutta-percha and pulp canal sealer incorporated with CS nanoparticles without surface treatment (group CS) or after surface treatment with phosphorylated CS (group PHCS), CS-conjugated rose bengal and photodynamic irradiation (group CSRB), or a combination of both PHCS and CSRB (group RBPH). The control group was filled with gutta-percha and an unmodified sealer. After 7 days of setting, specimens were aged in buffered solution at 37°C for 1 or 4 weeks. Monospecies biofilms of Enterococcus faecalis were grown on specimens for 7 days in a chemostat-based biofilm fermentor. Biofilm formation within the sealer-dentin interface was assessed with confocal laser scanning microscopy.
RESULTS: In the 4-week-aged specimens only, the mean biofilm areas were significantly smaller than in the control for the CS (P = .008), PHCS (P = .012), and RBPH (P = .034) groups. The percentage of the biofilm-covered interface also was significantly lower than in the control for the CS (P = .024) and PHCS (P = .003) groups. The CS, PHCS, and RBPH groups did not differ significantly.
CONCLUSIONS: Incorporating CS nanoparticles into the zinc oxide-eugenol sealer inhibited biofilm formation within the sealer-dentin interface. This effect was maintained when canals were treated with phosphorylated CS, and it was moderated by canal treatment with CS-conjugated rose bengal and irradiation.
Authors:
Luis DaSilva; Yoav Finer; Shimon Friedman; Bettina Basrani; Anil Kishen
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2012-12-17
Journal Detail:
Title:  Journal of endodontics     Volume:  39     ISSN:  1878-3554     ISO Abbreviation:  J Endod     Publication Date:  2013 Feb 
Date Detail:
Created Date:  2013-01-16     Completed Date:  2014-01-09     Revised Date:  2014-02-04    
Medline Journal Info:
Nlm Unique ID:  7511484     Medline TA:  J Endod     Country:  United States    
Other Details:
Languages:  eng     Pagination:  249-53     Citation Subset:  D    
Copyright Information:
Copyright © 2013 American Association of Endodontists. Published by Elsevier Inc. All rights reserved.
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MeSH Terms
Descriptor/Qualifier:
Animals
Anti-Infective Agents, Local / therapeutic use*
Biofilms / drug effects,  growth & development*
Buffers
Cattle
Chitosan / therapeutic use*
Collagen / drug effects
Dentin / drug effects,  microbiology*
Enterococcus faecalis / drug effects,  growth & development
Gutta-Percha / therapeutic use
Materials Testing
Microscopy, Confocal
Microscopy, Electron, Scanning
Nanoparticles / therapeutic use*
Photochemotherapy
Photosensitizing Agents / therapeutic use
Root Canal Filling Materials / therapeutic use*
Rose Bengal / therapeutic use
Surface Properties
Temperature
Time Factors
Tooth Root / drug effects,  microbiology*
Zinc Oxide-Eugenol Cement / therapeutic use
Grant Support
ID/Acronym/Agency:
MOP115113//Canadian Institutes of Health Research; R01 DE021385/DE/NIDCR NIH HHS; R01DE021385/DE/NIDCR NIH HHS
Chemical
Reg. No./Substance:
0/Anti-Infective Agents, Local; 0/Buffers; 0/Photosensitizing Agents; 0/Root Canal Filling Materials; 0/Zinc Oxide-Eugenol Cement; 1ZPG1ELY14/Rose Bengal; 9000-32-2/Gutta-Percha; 9007-34-5/Collagen; 9012-76-4/Chitosan
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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