Document Detail

Biofabrication of tissue constructs by 3D bioprinting of cell-laden microcarriers.
MedLine Citation:
PMID:  25048797     Owner:  NLM     Status:  Publisher    
Bioprinting allows the fabrication of living constructs with custom-made architectures by spatially controlled deposition of multiple bioinks. This is important for the generation of tissue, such as osteochondral tissue, which displays a zonal composition in the cartilage domain supported by the underlying subchondral bone. Challenges in fabricating functional grafts of clinically relevant size include the incorporation of cues to guide specific cell differentiation and the generation of sufficient cells, which is hard to obtain with conventional cell culture techniques. A novel strategy to address these demands is to combine bioprinting with microcarrier technology. This technology allows for the extensive expansion of cells, while they form multi-cellular aggregates, and their phenotype can be controlled. In this work, living constructs were fabricated via bioprinting of cell-laden microcarriers. Mesenchymal stromal cell (MSC)-laden polylactic acid microcarriers, obtained via static culture or spinner flask expansion, were encapsulated in gelatin methacrylamide-gellan gum bioinks, and the printability of the composite material was studied. This bioprinting approach allowed for the fabrication of constructs with high cell concentration and viability. Microcarrier encapsulation improved the compressive modulus of the hydrogel constructs, facilitated cell adhesion, and supported osteogenic differentiation and bone matrix deposition by MSCs. Bilayered osteochondral models were fabricated using microcarrier-laden bioink for the bone compartment. These findings underscore the potential of this new microcarrier-based biofabrication approach for bone and osteochondral constructs.
Riccardo Levato; Jetze Visser; Josep A Planell; Elisabeth Engel; Jos Malda; Miguel A Mateos-Timoneda
Related Documents :
24506867 - Pdx1 maintains β cell identity and function by repressing an α cell program.
24512567 - Radiation-induced mitotic catastrophe in fancd2 primary fibroblasts.
24749537 - Isoflavanones from desmodium oxyphyllum and their cytotoxicity.
24901747 - Optimization of absorption bands of dye-sensitized and perovskite tandem solar cells ba...
24665387 - Arhgef11, a regulator of junction-associated actomyosin in epithelial cells.
24506867 - Pdx1 maintains β cell identity and function by repressing an α cell program.
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2014-7-22
Journal Detail:
Title:  Biofabrication     Volume:  6     ISSN:  1758-5090     ISO Abbreviation:  Biofabrication     Publication Date:  2014 Jul 
Date Detail:
Created Date:  2014-7-22     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101521964     Medline TA:  Biofabrication     Country:  -    
Other Details:
Languages:  ENG     Pagination:  035020     Citation Subset:  -    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  A spatial analysis to study access to emergency obstetric transport services under the public privat...
Next Document:  Association of Increased Circulating Catecholamine and Glucocorticoid Levels with Risk of Psychologi...