Document Detail

Bioencapsulation of living bacteria (Escherichia coli) with poly(silicate) after transformation with silicatein-alpha gene.
MedLine Citation:
PMID:  18022688     Owner:  NLM     Status:  MEDLINE    
Bioencapsulation is an intriguing way to immobilize biological materials, including cells, in silica, metal-oxides or hybrid sol-gel polymers. Until now only the sol-gel precursor technology was utilized to immobilize bacteria or yeast cells in silica. With the discovery of silicatein, an enzyme from demosponges that catalyzes the formation of poly(silicate), it became possible to synthesize poly(silicate) under physiological (ambient) conditions. Here we show that Escherichia coli can be transformed with the silicatein gene, its expression level in the presence of isopropyl beta-D-thiogalactopyranoside (IPTG) can be efficiently intensified by co-incubation with silicic acid. This effect could be demonstrated on the level of recombinant protein synthesis as well as by immunostaining analysis. The heterologously produced silicatein is enzymatically active, as confirmed by staining with Rhodamine 123 (formation for poly[silicate] from silicic acid) and by reacting free silicic acid with the beta-silicomolybdato color system. Electron microscopic analysis revealed that the bacteria that express silicatein form a viscous cover around them when growing in the presence of silicic acid. Finally, we demonstrate that the growth kinetics of E. coli remains unaffected whether or not the bacteria had been transformed with silicatein or grown in medium, supplemented with silicic acid. It is concluded that silicatein-mediated encapsulation of bacteria with silica might improve, extend and optimize the range of application of bacteria for the production of recombinant protein.
Werner E G Müller; Sylvia Engel; Xiaohong Wang; Stephan E Wolf; Wolfgang Tremel; Narsinh L Thakur; Anatoli Krasko; Mugdha Divekar; Heinz C Schröder
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-11-26
Journal Detail:
Title:  Biomaterials     Volume:  29     ISSN:  0142-9612     ISO Abbreviation:  Biomaterials     Publication Date:  2008 Mar 
Date Detail:
Created Date:  2007-12-24     Completed Date:  2008-02-29     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8100316     Medline TA:  Biomaterials     Country:  England    
Other Details:
Languages:  eng     Pagination:  771-9     Citation Subset:  IM    
Institut für Physiologische Chemie, Abteilung Angewandte Molekularbiologie, Universität, Duesbergweg 6, D-55099 Mainz, Germany.
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MeSH Terms
Bacterial Capsules / chemistry*
Cathepsins / genetics*,  metabolism*
Escherichia coli* / cytology,  genetics,  metabolism
Gene Expression
Microbial Viability
Microscopy, Electron, Scanning
Silicates / chemistry*
Sodium / chemistry
Transgenes / genetics*
Reg. No./Substance:
0/Bacterial Capsules; 0/Silicates; 0/silicatein alpha; 7440-23-5/Sodium; EC 3.4.-/Cathepsins

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