| Bioelectrochemical control of neural cell development on conducting polymers. | |
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MedLine Citation:
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PMID: 20864170 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Electrically conducting polymers hold promise for developing advanced neuroprostheses, bionic systems and neural repair devices. Among them, poly(3, 4-ethylenedioxythiophene) doped with polystyrene sulfonate (PEDOT:PSS) exhibits superior physicochemical properties but biocompatibility issues have limited its use. We describe combinations of electrochemical and molecule self-assembling methods to consistently control neural cell development on PEDOT:PSS while maintaining very low interfacial impedance. Electro-adsorbed polylysine enabled long-term neuronal survival and growth on the nanostructured polymer. Neurite extension was strongly inhibited by an additional layer of PSS or heparin, which in turn could be either removed electrically or further coated with spermine to activate cell growth. Binding basic fibroblast growth factor (bFGF) to the heparin layer inhibited neurons but promoted proliferation and migration of precursor cells. This methodology may orchestrate neural cell behavior on electroactive polymers, thus improving cell/electrode communication in prosthetic devices and providing a platform for tissue repair strategies. |
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Authors:
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Jorge E Collazos-Castro; José L Polo; Gabriel R Hernández-Labrado; Vanesa Padial-Cañete; Concepción García-Rama |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-09-22 |
Journal Detail:
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Title: Biomaterials Volume: 31 ISSN: 1878-5905 ISO Abbreviation: Biomaterials Publication Date: 2010 Dec |
Date Detail:
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Created Date: 2010-10-18 Completed Date: 2011-01-27 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8100316 Medline TA: Biomaterials Country: England |
Other Details:
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Languages: eng Pagination: 9244-55 Citation Subset: IM |
Copyright Information:
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Copyright © 2010 Elsevier Ltd. All rights reserved. |
Affiliation:
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Neural Repair Laboratory, Hospital Nacional de Parapléjicos, Finca La Peraleda s/n, Toledo, Spain. jcollazos@sescam.org |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Bicyclo Compounds, Heterocyclic / chemistry, pharmacology Cell Movement / drug effects Cell Proliferation / drug effects Cells, Cultured Electrochemistry* Fibroblast Growth Factor 2 / chemistry, pharmacology Heparin / chemistry, pharmacology Microscopy, Electron Neurons / cytology*, drug effects, metabolism Polymers / chemistry*, pharmacology Polystyrenes / chemistry, pharmacology Rats Rats, Wistar Spermine / chemistry, pharmacology |
| Chemical | |
Reg. No./Substance:
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0/Bicyclo Compounds, Heterocyclic; 0/Polymers; 0/Polystyrenes; 0/poly(3,4-ethylene dioxythiophene); 103107-01-3/Fibroblast Growth Factor 2; 28210-41-5/polystyrene sulfonic acid; 71-44-3/Spermine; 9005-49-6/Heparin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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