Document Detail


Bioelectrochemical control of neural cell development on conducting polymers.
MedLine Citation:
PMID:  20864170     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Electrically conducting polymers hold promise for developing advanced neuroprostheses, bionic systems and neural repair devices. Among them, poly(3, 4-ethylenedioxythiophene) doped with polystyrene sulfonate (PEDOT:PSS) exhibits superior physicochemical properties but biocompatibility issues have limited its use. We describe combinations of electrochemical and molecule self-assembling methods to consistently control neural cell development on PEDOT:PSS while maintaining very low interfacial impedance. Electro-adsorbed polylysine enabled long-term neuronal survival and growth on the nanostructured polymer. Neurite extension was strongly inhibited by an additional layer of PSS or heparin, which in turn could be either removed electrically or further coated with spermine to activate cell growth. Binding basic fibroblast growth factor (bFGF) to the heparin layer inhibited neurons but promoted proliferation and migration of precursor cells. This methodology may orchestrate neural cell behavior on electroactive polymers, thus improving cell/electrode communication in prosthetic devices and providing a platform for tissue repair strategies.
Authors:
Jorge E Collazos-Castro; José L Polo; Gabriel R Hernández-Labrado; Vanesa Padial-Cañete; Concepción García-Rama
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-09-22
Journal Detail:
Title:  Biomaterials     Volume:  31     ISSN:  1878-5905     ISO Abbreviation:  Biomaterials     Publication Date:  2010 Dec 
Date Detail:
Created Date:  2010-10-18     Completed Date:  2011-01-27     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8100316     Medline TA:  Biomaterials     Country:  England    
Other Details:
Languages:  eng     Pagination:  9244-55     Citation Subset:  IM    
Copyright Information:
Copyright © 2010 Elsevier Ltd. All rights reserved.
Affiliation:
Neural Repair Laboratory, Hospital Nacional de Parapléjicos, Finca La Peraleda s/n, Toledo, Spain. jcollazos@sescam.org
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MeSH Terms
Descriptor/Qualifier:
Animals
Bicyclo Compounds, Heterocyclic / chemistry,  pharmacology
Cell Movement / drug effects
Cell Proliferation / drug effects
Cells, Cultured
Electrochemistry*
Fibroblast Growth Factor 2 / chemistry,  pharmacology
Heparin / chemistry,  pharmacology
Microscopy, Electron
Neurons / cytology*,  drug effects,  metabolism
Polymers / chemistry*,  pharmacology
Polystyrenes / chemistry,  pharmacology
Rats
Rats, Wistar
Spermine / chemistry,  pharmacology
Chemical
Reg. No./Substance:
0/Bicyclo Compounds, Heterocyclic; 0/Polymers; 0/Polystyrenes; 0/poly(3,4-ethylene dioxythiophene); 103107-01-3/Fibroblast Growth Factor 2; 28210-41-5/polystyrene sulfonic acid; 71-44-3/Spermine; 9005-49-6/Heparin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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