Document Detail


Biodistribution of the radiophamarceutical sodium pertechnetate (Na99mTcO4) after massive small bowel resection in rats.
MedLine Citation:
PMID:  18235929     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
PURPOSE: To evaluate the biodistribution of sodium pertecnetate (Na(99m)TcO(4)) in organs and tissues, the morphometry of remnant intestinal mucosa and ponderal evolution in rats subjected to massive resection of the small intestine. METHODS: Twenty-one Wistar rats were randomly divided into three groups of 7 animals each. The short bowel (SB) group was subjected to massive resection of the small intestine; the control group (C) rats were not operated on, and soft intestinal handling was performed in sham rats. The animals were weighed weekly. On the 30th postoperative day, 0.l mL of Na(99m)TcO(4), with mean activity of 0.66 MBq was injected intravenously into the orbital plexus. After 30 minutes, the rats were killed with an overdose of anesthetic, and fragments of the liver, spleen, pancreas, stomach, duodenum, small intestine, thyroid, lung, heart, kidney, bladder, muscle, femur and brain were harvested. The biopsies were washed with 0.9% NaCl.,The radioactivity was counted using Gama Counter Wizard 1470, PerkinElmer. The percentage of radioactivity per gram of tissue (%ATI/g) was calculated. Biopsies of the remaining jejunum were analysed by HE staining to obtain mucosal thickness. Analysis of variance (ANOVA) and the Tukey test for multiple comparisons were used, considering p<0.05 as significant. RESULTS: There were no significant differences in %ATI/g of the Na(99m)TcO(4) in the organs of the groups studied (p>0.05). An increase in the weight of the SB rats was observed after the second postoperative week. The jejunal mucosal thickness of the SB rats was significantly greater than that of C and sham rats (p<0.05). CONCLUSION: In rats with experimentally-produced short bowel syndrome, an adaptive response by the intestinal mucosa reduced weight loss. The biodistribution of Na(99m)TcO(4) was not affected by massive intestinal resection, suggesting that short bowel syndrome is not the cause of misleading interpretation, if an examination using this radiopharmaceutical is indicated.
Authors:
Dâmaso de Araújo Chacon; Irami Araújo-Filho; Arthur Villarim-Neto; Amália Cínthia Meneses Rêgo; Italo Medeiros Azevedo; Mário Bernardo-Filho; José Brandão-Neto; Aldo Cunha Medeiros
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Acta cirúrgica brasileira / Sociedade Brasileira para Desenvolvimento Pesquisa em Cirurgia     Volume:  22     ISSN:  0102-8650     ISO Abbreviation:  Acta Cir Bras     Publication Date:    2007 Nov-Dec
Date Detail:
Created Date:  2008-01-31     Completed Date:  2009-03-16     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9103983     Medline TA:  Acta Cir Bras     Country:  Brazil    
Other Details:
Languages:  eng     Pagination:  430-5     Citation Subset:  IM    
Affiliation:
Postgraduate Program in Health Sciences, UFRN, Brazil. damasochacon@uol.com.br
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MeSH Terms
Descriptor/Qualifier:
Animals
Disease Models, Animal
Intestinal Mucosa / pathology
Intestine, Small / pathology,  surgery*
Radiopharmaceuticals / pharmacokinetics*
Random Allocation
Rats
Rats, Wistar
Short Bowel Syndrome / metabolism*
Sodium Pertechnetate Tc 99m / pharmacokinetics*
Tissue Distribution
Chemical
Reg. No./Substance:
0/Radiopharmaceuticals; 23288-60-0/Sodium Pertechnetate Tc 99m

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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