| Biodistribution and catabolism of 18F-labelled isopeptide N(epsilon)-(gamma-glutamyl)-L-lysine. | |
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MedLine Citation:
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PMID: 15924212 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Isopeptide bonds between the epsilon-amino group of lysine and the gamma-carboxamide group of glutamine are formed during strong heating of pure proteins or, more important, by enzymatic reaction mediated by transglutaminases. Despite the wide use of a microbial transglutaminase in food biotechnology, up to now little is known about the metabolic fate of the isopeptide N(epsilon)-(gamma-glutamyl)-L-lysine. In the present study, N-succinimidyl-4-[(18)F]fluorobenzoate was used to modify N(epsilon)-(gamma-glutamyl)-L-lysine at each of its two alpha-amino groups, resulting in the 4-[(18)F]fluorobenzoylated derivatives, for which biodistribution, catabolism, and elimination were investigated in male Wistar rats. A significant different biochemical behavior of the two labelled isopeptides was observed in terms of in vitro stability, in vivo metabolism as well as biodistribution. The results suggest that the metabolic fate of isopeptides is likely to be dependent on how they are reabsorbed - free or peptide bound. |
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Authors:
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C Hultsch; R Bergmann; B Pawelke; J Pietzsch; F Wuest; B Johannsen; T Henle |
Publication Detail:
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Type: In Vitro; Journal Article Date: 2005-05-31 |
Journal Detail:
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Title: Amino acids Volume: 29 ISSN: 0939-4451 ISO Abbreviation: Amino Acids Publication Date: 2005 Dec |
Date Detail:
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Created Date: 2005-12-19 Completed Date: 2006-08-28 Revised Date: 2008-12-15 |
Medline Journal Info:
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Nlm Unique ID: 9200312 Medline TA: Amino Acids Country: Austria |
Other Details:
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Languages: eng Pagination: 405-13 Citation Subset: IM |
Affiliation:
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Positron Emission Tomography Center, Institute of Bioinorganic and Radiopharmaceutical Chemistry, Research Center Rossendorf, Dresden, Germany. ch.hultsch@fz-rossendorf.de |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Dipeptides / chemistry, metabolism*, pharmacokinetics* Fluorine Radioisotopes Isotope Labeling / methods Male Molecular Structure Positron-Emission Tomography / methods Rats Rats, Wistar Sensitivity and Specificity |
| Chemical | |
Reg. No./Substance:
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0/Dipeptides; 0/Fluorine Radioisotopes; 17105-15-6/epsilon-(gamma-glutamyl)-lysine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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