Document Detail


Biodegradation of triclosan by a wastewater microorganism.
MedLine Citation:
PMID:  22673343     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Triclosan, a synthetic antimicrobial agent, has been considered as an emerging environmental contaminant. Here we reported a triclosan-degrading wastewater bacterial isolate, Sphingopyxis strain KCY1, capable of dechlorinating triclosan with a stoichiometric release of chloride. The stain can degrade diphenyl ether but not 2,4,4'-tribromodiphenyl ether and 2,2',4,4'-tetrabromodiphenyl ether, despite all these three compounds are structurally similar to triclosan. While strain KCY1 was unable to grow on triclosan and catechol, it could grow with glucose, sodium succinate, sodium acetate, and phenol. When grown with complex nutrient medium containing a trace amount of triclosan (as low as 5 μg/L), the strain could retain its degradation ability toward triclosan. The maximum-specific triclosan degradation rate (q(m)) and the half-velocity constant (K(m)) are 0.13 mg-triclosan/mg-protein/day and 2.8 mg-triclosan/L, respectively. As triclosan degradation progressed, five metabolites were identified and these metabolites continue to transform into non-chlorinated end products, which was supported by a sharp drop in androgenic potential. The activity of catechol 2,3-dioxygenase in the cell extract was detected. No triclosan degradation was observed in the presence of 3-fluorocatechol, an inhibitor of meta-cleavage enzyme, suggesting that triclosan degradation proceed via meta-cleavage pathway. Based on all the observations, a degradation pathway for triclosan by strain KCY1 was proposed.
Authors:
Do Gyun Lee; Fuman Zhao; Yohannes H Rezenom; David H Russell; Kung-Hui Chu
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Publication Detail:
Type:  Journal Article     Date:  2012-05-21
Journal Detail:
Title:  Water research     Volume:  46     ISSN:  1879-2448     ISO Abbreviation:  Water Res.     Publication Date:  2012 Sep 
Date Detail:
Created Date:  2012-06-27     Completed Date:  2012-10-30     Revised Date:  2013-08-12    
Medline Journal Info:
Nlm Unique ID:  0105072     Medline TA:  Water Res     Country:  England    
Other Details:
Languages:  eng     Pagination:  4226-34     Citation Subset:  IM    
Copyright Information:
Copyright © 2012 Elsevier Ltd. All rights reserved.
Affiliation:
Department of Civil Engineering, Texas A&M University, 205G WERC, 3136 TAMU College Station, TX 77843-3136, USA.
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MeSH Terms
Descriptor/Qualifier:
Biodegradation, Environmental
Catechol 2,3-Dioxygenase / metabolism
Catechols / chemistry,  metabolism
Chlorides / chemistry,  metabolism
Glucose / metabolism
Kinetics
Models, Chemical
Molecular Structure
Phenol / metabolism
Phenyl Ethers / chemistry,  metabolism
Polybrominated Biphenyls / chemistry,  metabolism
Sodium Acetate / metabolism
Sphingomonadaceae / growth & development,  isolation & purification,  metabolism*
Succinates / metabolism
Triclosan / chemistry,  metabolism*
Water Microbiology*
Water Pollutants, Chemical / chemistry,  metabolism*
Chemical
Reg. No./Substance:
0/Catechols; 0/Chlorides; 0/Phenyl Ethers; 0/Polybrominated Biphenyls; 0/Succinates; 0/Water Pollutants, Chemical; 0N97R5X10X/2,2',4,4'-tetrabromodiphenyl ether; 108-95-2/Phenol; 3380-34-5/Triclosan; 3O695R5M1U/phenyl ether; 50-99-7/Glucose; 6131-90-4/Sodium Acetate; EC 1.13.11.2/Catechol 2,3-Dioxygenase; LF3AJ089DQ/catechol

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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