Document Detail


Biodegradation of sulfanilic acid by Pseudomonas paucimobilis.
MedLine Citation:
PMID:  11234949     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
An aerobic bacterium, isolated from a contaminated site, was able to degrade sulfanilic acid (4-aminobenzenesulfonic acid) and was identified as Pseudomonas paucimobilis. The isolate could grow on sulfanilic acid (SA) as its sole carbon and nitrogen source and metabolized the target compound to biomass. The bioconversion capacity depended on the sulfanilic acid concentration; greater than 98% elimination of the hazardous compound was achieved at low (10 mM) sulfanilic acid concentration, and the yield was greater than 70% at 50 mM concentration of the contaminant. The maximum conversion rate was 1.5 mmol sulfanilic acid/h per mg wet cells at 30 degrees C. Ca-alginate-phytagel proved a good matrix for immobilization of P. paucimobilis, with essentially unaltered biodegradation activity. Removal of sulfanilic acid from contaminated industrial waste water was demonstrated. SDS-PAGE analysis of the crude extract revealed novel proteins appearing upon induction with sulfanilic acid and related compounds, which indicated alternative degradation mechanisms involving various inducible enzymes.
Authors:
K Perei; G Rákhely; I Kiss; B Polyák; K L Kovács
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Applied microbiology and biotechnology     Volume:  55     ISSN:  0175-7598     ISO Abbreviation:  Appl. Microbiol. Biotechnol.     Publication Date:  2001 Jan 
Date Detail:
Created Date:  2001-03-07     Completed Date:  2001-07-12     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8406612     Medline TA:  Appl Microbiol Biotechnol     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  101-7     Citation Subset:  IM    
Affiliation:
Institute of Biophysics, Biological Research Center, Hungarian Academy of Sciences, Szeged.
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MeSH Terms
Descriptor/Qualifier:
Biodegradation, Environmental
Biotechnology / methods
Cells, Immobilized
Culture Media
Pseudomonas / growth & development,  metabolism*
Substrate Specificity
Sulfanilic Acids / metabolism*
Waste Disposal, Fluid / methods
Water Microbiology
Water Pollution, Chemical
Water Purification / methods
Chemical
Reg. No./Substance:
0/Culture Media; 0/Sulfanilic Acids

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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