Document Detail

Biodegradation of cyanides, cyanates and thiocyanates to ammonia and carbon dioxide by immobilized cells of Pseudomonas putida.
MedLine Citation:
PMID:  9523454     Owner:  NLM     Status:  MEDLINE    
Pseudomonas putida utilizes cyanide as the sole source of carbon and nitrogen. Agar, alginate, and carrageenan were screened as the encapsulating matrices for P. putida. Alginate-immobilized cells of P. putida degraded sodium cyanide (NaCN) more efficiently than non-immobilized cells or cells immobilized in agar or carrageenan. The end products of biodegradation of cyanide were identified as ammonia (NH3) and carbon dioxide (CO2). These products changed the medium pH. In bioreactors, the rate of cyanide degradation increased with an increase in the rate of aeration. Maximum utilization of cyanide was observed at 200 ml min-1 of aeration. Immobilized cells of P. putida degraded cyanides, cyanates and thiocyanates to NH3 and CO2. Use of Na[14C]-CN showed that 70% of carbon of Na[14C]-CN was converted into 14CO2 and only 10% was associated with the cell biomass. The substrate-dependent kinetics indicated that the Km and Vmax values of P. putida for the substrate, NaCN were 14 mM and 29 nmol of oxygen consumed mg protein-1 min-1 respectively.
K D Chapatwala; G R Babu; O K Vijaya; K P Kumar; J H Wolfram
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of industrial microbiology & biotechnology     Volume:  20     ISSN:  1367-5435     ISO Abbreviation:  J. Ind. Microbiol. Biotechnol.     Publication Date:  1998 Jan 
Date Detail:
Created Date:  1998-04-29     Completed Date:  1998-04-29     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9705544     Medline TA:  J Ind Microbiol Biotechnol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  28-33     Citation Subset:  B    
Division of Natural Sciences, Selma University, AL 36701, USA.
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MeSH Terms
Agar / chemistry
Alginates / chemistry
Ammonia / chemistry*
Biodegradation, Environmental
Carbon Dioxide / chemistry*
Carrageenan / chemistry
Cells, Immobilized
Culture Media / chemistry
Cyanates / metabolism*
Cyanides / metabolism*
Excipients / chemistry
Pseudomonas putida / growth & development,  metabolism*
Thiocyanates / metabolism*
Reg. No./Substance:
0/Alginates; 0/Culture Media; 0/Cyanates; 0/Cyanides; 0/Excipients; 0/Thiocyanates; 124-38-9/Carbon Dioxide; 7664-41-7/Ammonia; 9000-07-1/Carrageenan; 9002-18-0/Agar

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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