Document Detail


Biodegradation of crystal violet by the white rot fungus Phanerochaete chrysosporium.
MedLine Citation:
PMID:  3389809     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Biodegradation of crystal violet (N,N,N',N',N'',N''-hexamethylpararosaniline) in ligninolytic (nitrogen-limited) cultures of the white rot fungus Phanerochaete chrysosporium was demonstrated by the disappearance of crystal violet and by the identification of three metabolites (N,N,N',N',N''-pentamethylpararosaniline, N,N,N',N''-tetramethylpararosaniline, and N,N',N''-trimethylpararosaniline) formed by sequential N-demethylation of the parent compound. Metabolite formation also occurred when crystal violet was incubated with the extracellular fluid obtained from ligninolytic cultures of this fungus, provided that an H2O2-generating system was supplied. This, as well as the fact that a purified ligninase catalyzed N-demethylation of crystal violet, demonstrated that biodegradation of crystal violet by this fungus is dependent, at least in part, upon its lignin-degrading system. In addition to crystal violet, six other triphenylmethane dyes (pararosaniline, cresol red, bromphenol blue, ethyl violet, malachite green, and brilliant green) were shown to be degraded by the lignin-degrading system of this fungus. An unexpected result was the finding that substantial degradation of crystal violet also occurred in nonligninolytic (nitrogen-sufficient) cultures of P. chrysosporium, suggesting that in addition to the lignin-degrading system, another mechanism exists in this fungus which is also able to degrade crystal violet.
Authors:
J A Bumpus; B J Brock
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Applied and environmental microbiology     Volume:  54     ISSN:  0099-2240     ISO Abbreviation:  Appl. Environ. Microbiol.     Publication Date:  1988 May 
Date Detail:
Created Date:  1988-07-29     Completed Date:  1988-07-29     Revised Date:  2010-09-09    
Medline Journal Info:
Nlm Unique ID:  7605801     Medline TA:  Appl Environ Microbiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1143-50     Citation Subset:  IM    
Affiliation:
Biology Department, Utah State University, Logan 84322-4430.
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MeSH Terms
Descriptor/Qualifier:
Basidiomycota / enzymology,  physiology*
Biodegradation, Environmental
Catalysis
Extracellular Space / microbiology,  physiology
Gentian Violet / analysis*
Lignin / metabolism
Mass Spectrometry
Oxidoreductases, N-Demethylating / metabolism
Oxygenases / physiology
Peroxidases*
Trityl Compounds / analysis
Chemical
Reg. No./Substance:
0/Trityl Compounds; 548-62-9/Gentian Violet; 9005-53-2/Lignin; EC 1.11.1.-/Peroxidases; EC 1.11.1.-/lignin peroxidase; EC 1.13.-/Oxygenases; EC 1.5.-/Oxidoreductases, N-Demethylating
Comments/Corrections

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