Document Detail

Biodegradable and synthetic membranes for the expansion and functional differentiation of rat embryonic liver cells.
MedLine Citation:
PMID:  20691288     Owner:  NLM     Status:  In-Process    
The insufficient availability of donor organs for orthotopic liver transplantation worldwide has urgently increased the requirement for new therapies for acute and chronic liver disease. The creation of an unlimited source of donor cells for hepatocyte transplantation therapy and pharmaceutical applications may be the isolation and expansion of liver progenitor cells or stem cells. Here we report the expansion and functional differentiation of rat embryonic liver cells on biodegradable and synthetic polymeric membranes in comparison with traditional substrates, such as collagen and polystyrene culture dishes. Membranes prepared from chitosan and modified polyetheretherketone were used for the culture of liver progenitor cells derived from rat embryonic liver. Cells proliferated, with a significant increase in their number within 8-11 days. The cells displayed functional differentiation showing urea synthesis, albumin production and diazepam biotransformation on all substrates investigated. In particular, on a chitosan membrane liver-specific functions were expressed at significantly higher levels for prolonged times compared with other synthetic membranes, utilizing traditional substrates (collagen and PSCD) as references. These results demonstrate that chitosan membranes offer cells favourable conditions to promote the expansion and functional differentiation of embryonic liver cells that could be effectively used in liver tissue engineering and in pharmaceutical applications.
Antonella Piscioneri; Carla Campana; Simona Salerno; Sabrina Morelli; Augustinus Bader; Francesca Giordano; Enrico Drioli; Loredana De Bartolo
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-08-04
Journal Detail:
Title:  Acta biomaterialia     Volume:  7     ISSN:  1878-7568     ISO Abbreviation:  Acta Biomater     Publication Date:  2011 Jan 
Date Detail:
Created Date:  2010-11-01     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101233144     Medline TA:  Acta Biomater     Country:  England    
Other Details:
Languages:  eng     Pagination:  171-9     Citation Subset:  IM    
Copyright Information:
Copyright © 2010 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.
Institute on Membrane Technology, ITM-CNR, University of Calabria, Rende, CS, Italy.
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