| Biodegradable magnetic-fluorescent magnetite/poly(dl-lactic acid-co-alpha,beta-malic acid) composite nanoparticles for stem cell labeling. | |
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MedLine Citation:
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PMID: 20144844 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Bifunctional superparamagnetic magnetite/poly(dl-lactic acid-co-alpha,beta-malic acid) composite nanoparticles (PLMA-MNPs) detectable by both magnetic resonance imaging (MRI) and fluorescence microscopy were synthesized by coating Fe(3)O(4) nanoparticles with biodegradable poly(dl-lactic acid-co-alpha,beta-malic acid) copolymer (PLMA) with covalently bound fluorescein isothiocyanate (FITC). The FITC modified PLMA-MNPs (FITC-PLMA-MNPs) have a hydrodynamic diameter of 100nm and an anionic surface. MTT assays of mouse macrophages, 3T3 fibroblasts and human mesenchymal stem cells (hMSCs) incubated with these nanoparticles indicated that these nanoparticles did not possess significant cytotoxicity. Furthermore, the osteogenic and adipogenic differentiations of the hMSCs were not influenced by the labeling process. As a result of the high R(2) (164.8mm(-1)s(-1)) and R(2)/R(1) ratio (32) of the FITC-PLMA-MNPs, the labeled hMSCs can be detected by a clinical 3T MRI scanner at an in vitro detection threshold of about 1200 cells. The green fluorescence associated with the FITC can be readily observed. Such nanoparticles can potentially be used as a T(2)-weighted contrast agent and fluorescent agent for stem cell labeling. |
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Authors:
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Liang Wang; Koon-Gee Neoh; En-Tang Kang; Borys Shuter; Shih-Chang Wang |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-02-09 |
Journal Detail:
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Title: Biomaterials Volume: 31 ISSN: 1878-5905 ISO Abbreviation: Biomaterials Publication Date: 2010 May |
Date Detail:
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Created Date: 2010-03-11 Completed Date: 2010-08-02 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8100316 Medline TA: Biomaterials Country: England |
Other Details:
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Languages: eng Pagination: 3502-11 Citation Subset: IM |
Copyright Information:
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Copyright 2010 Elsevier Ltd. All rights reserved. |
Affiliation:
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Department of Diagnostic Radiology, National University of Singapore, Kent Ridge, Singapore 119260, Singapore. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Differentiation Cells, Cultured Fluorescent Dyes / chemistry* Humans Magnetic Resonance Spectroscopy Magnetics* Mice Microscopy, Confocal Microscopy, Electron, Transmission Nanoparticles* Polyesters / metabolism* Spectroscopy, Fourier Transform Infrared Stem Cells / cytology* |
| Chemical | |
Reg. No./Substance:
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0/Fluorescent Dyes; 0/Polyesters; 0/poly(lactic acid-co-malic acid) |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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