Document Detail


Bioconversion of linoleic acid into conjugated linoleic acid by immobilized Lactobacillus reuteri.
MedLine Citation:
PMID:  12790685     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Lactobacillus reuteri was immobilized on silica gel to evaluate the bioconversion of linoleic acid (LA) into conjugated linoleic acid (CLA), consisting of cis-9,trans-11 and trans-10,cis-12 isomers. The amount of cell to carrier, the reaction time, and the substrate concentration, pH, and temperature for CLA production were optimized at 10 mg of cells/(g of carrier), 1 h, 500 mg/L LA, 10.5, and 55 degrees C, respectively. In the presence of 1.0 mM Cu(2+), CLA production increased by 110%. Under the optimal conditions, the immobilized cells produced 175 mg/L CLA from 500 mg/L LA for 1 h with a productivity of 175 mg/(L.h) and accumulated 5.5 times more CLA than that obtained from bioconversion by free washed cells. The CLA-producing ability of reused cells was investigated over five reuse reactions and was maximal at pH 7.5, 25 degrees C, and 1.0 mM Cu(2+). The total amount of CLA by the combined five reuse reactions was 344 mg of CLA/L reaction volume. This was 8.6 times higher than the amount obtained from reuse reactions by free washed cells.
Authors:
Sun-Ok Lee; Geun-Wha Hong; Deok-Kun Oh
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Publication Detail:
Type:  Comparative Study; Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biotechnology progress     Volume:  19     ISSN:  8756-7938     ISO Abbreviation:  Biotechnol. Prog.     Publication Date:    2003 May-Jun
Date Detail:
Created Date:  2003-06-06     Completed Date:  2004-05-24     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8506292     Medline TA:  Biotechnol Prog     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1081-4     Citation Subset:  IM    
Affiliation:
Department of Bioscience and Biotechnology, Sejong University, Seoul 143-747, Korea.
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MeSH Terms
Descriptor/Qualifier:
Bioreactors / microbiology*
Biotransformation
Cell Culture Techniques / methods*
Cells, Immobilized / physiology
Copper / metabolism
Hydrogen-Ion Concentration
Lactobacillus / classification,  growth & development*,  metabolism*
Linoleic Acid / metabolism*
Linoleic Acids, Conjugated / biosynthesis*
Temperature
Chemical
Reg. No./Substance:
0/Linoleic Acids, Conjugated; 2197-37-7/Linoleic Acid; 7440-50-8/Copper

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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