| Bioconversion of glycyrrhizinic acid in liquorice into 18-beta-glycyrrhetinic acid by Aspergillus parasiticus speare BGB. | |
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MedLine Citation:
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PMID: 20873172 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A filamentous fungi strain, Aspergillus parasiticus Speare BGB, producing beta-glucuronidase was screened to transform glycyrrhizinic acid (GL) in liquorice into 18-beta-glycyrrhetinic acid (GA). Under the following cultivate conditions in shake flask, 1% GL (purity 30%), medium capacity 40% of flask, the initial pH value at 4.5, cultivate temperature of 32 degrees C, inoculum size of 5% and culturing time for 96 h the bioconversion ratio of GL into GA could reach 95%. A variety of parameters of submerged state fermentation, including the growth characteristics of A. parasiticus Speare BGB, the change amount of GL and GA, and the activity of beta-glucuronidase, were monitored simultaneously. GA was separated and purified by macroporous resin, silica gel column chromatography followed by recrystalization with the final purity over 98%. Purified product was identified as GA by the infrared absorption spectrum, molecular weight, and nuclear magnetic resonance. This study provided a new and efficient approach of obtaining GA by microbial transformation. |
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Authors:
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J Wang; Q Sun; P Gao; J F Wang; C Xu; Q L Sun |
Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Prikladnaia biokhimiia i mikrobiologiia Volume: 46 ISSN: 0555-1099 ISO Abbreviation: Prikl. Biokhim. Mikrobiol. Publication Date: 2010 Jul-Aug |
Date Detail:
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Created Date: 2010-09-28 Completed Date: 2010-11-12 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0023416 Medline TA: Prikl Biokhim Mikrobiol Country: Russia (Federation) |
Other Details:
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Languages: eng Pagination: 462-6 Citation Subset: IM |
Affiliation:
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College of Life Sciences, Key Laboratory of Bio-resources and Eco-environment of Ministry of Education, Sichuan University Chengdu 610064, PR China. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Aspergillus
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enzymology*,
isolation & purification* Fermentation Glycyrrhetinic Acid / metabolism* Glycyrrhiza uralensis / microbiology* Glycyrrhizic Acid / metabolism* |
| Chemical | |
Reg. No./Substance:
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1405-86-3/Glycyrrhizic Acid; 471-53-4/Glycyrrhetinic Acid |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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