Document Detail

Bioconversion of glycyrrhizinic acid in liquorice into 18-beta-glycyrrhetinic acid by Aspergillus parasiticus speare BGB.
MedLine Citation:
PMID:  20873172     Owner:  NLM     Status:  MEDLINE    
A filamentous fungi strain, Aspergillus parasiticus Speare BGB, producing beta-glucuronidase was screened to transform glycyrrhizinic acid (GL) in liquorice into 18-beta-glycyrrhetinic acid (GA). Under the following cultivate conditions in shake flask, 1% GL (purity 30%), medium capacity 40% of flask, the initial pH value at 4.5, cultivate temperature of 32 degrees C, inoculum size of 5% and culturing time for 96 h the bioconversion ratio of GL into GA could reach 95%. A variety of parameters of submerged state fermentation, including the growth characteristics of A. parasiticus Speare BGB, the change amount of GL and GA, and the activity of beta-glucuronidase, were monitored simultaneously. GA was separated and purified by macroporous resin, silica gel column chromatography followed by recrystalization with the final purity over 98%. Purified product was identified as GA by the infrared absorption spectrum, molecular weight, and nuclear magnetic resonance. This study provided a new and efficient approach of obtaining GA by microbial transformation.
J Wang; Q Sun; P Gao; J F Wang; C Xu; Q L Sun
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Prikladnaia biokhimiia i mikrobiologiia     Volume:  46     ISSN:  0555-1099     ISO Abbreviation:  Prikl. Biokhim. Mikrobiol.     Publication Date:    2010 Jul-Aug
Date Detail:
Created Date:  2010-09-28     Completed Date:  2010-11-12     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0023416     Medline TA:  Prikl Biokhim Mikrobiol     Country:  Russia (Federation)    
Other Details:
Languages:  eng     Pagination:  462-6     Citation Subset:  IM    
College of Life Sciences, Key Laboratory of Bio-resources and Eco-environment of Ministry of Education, Sichuan University Chengdu 610064, PR China.
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MeSH Terms
Aspergillus / enzymology*,  isolation & purification*
Glycyrrhetinic Acid / metabolism*
Glycyrrhiza uralensis / microbiology*
Glycyrrhizic Acid / metabolism*
Reg. No./Substance:
1405-86-3/Glycyrrhizic Acid; 471-53-4/Glycyrrhetinic Acid

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