Document Detail


Bioconversion of a L-carnitin precursor in a one- or two-phase system.
MedLine Citation:
PMID:  1929377     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The ability of the yeast Saccharomyces cerevisiae to bioconvert stereo-selectively octyl-4-chloroacetoacetate (OCA) into the corresponding chiral alcohol, precursor of L-carnitin, an important physiological agent, was investigated. In a monophasic system with free cells, more than 90% of OCA (0.018 M) bioconversion have been reached after 6 h (enantiomeric excess for the R form, eeR:97%). Immobilized cells in alginate beads were less efficient in conversion of OCA than free cells. In a two-phase system with free cells, the level of reduction of OCA (0.018 M) reached 85% after 48 h. With a medium containing a higher OCA concentration (0.270 M), 41% of this product were bioconverted after the same period. On the other hand, immobilized cells did not show any significant bioconversion of OCA in two-phase reactors. The limiting factor of these reactors in the regeneration of the cofactors involved in the OCA reduction.
Authors:
G Bare; P Jacques; J B Hubert; R Rikir; P Thonart
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Applied biochemistry and biotechnology     Volume:  28-29     ISSN:  0273-2289     ISO Abbreviation:  Appl. Biochem. Biotechnol.     Publication Date:  1991  
Date Detail:
Created Date:  1991-10-31     Completed Date:  1991-10-31     Revised Date:  2000-12-18    
Medline Journal Info:
Nlm Unique ID:  8208561     Medline TA:  Appl Biochem Biotechnol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  445-56     Citation Subset:  IM    
Affiliation:
Centre Wallon de Biologie Industrielle, Université de Liège, Belgium.
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MeSH Terms
Descriptor/Qualifier:
Acetoacetates / metabolism*
Biotransformation
Carnitine / biosynthesis*
Kinetics
Saccharomyces cerevisiae / growth & development,  metabolism*
Chemical
Reg. No./Substance:
0/Acetoacetates; 41051-21-2/octyl-4-chloroacetoacetate; 541-15-1/Carnitine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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