| Biocompatible and biodegradable polymersome encapsulated hemoglobin: a potential oxygen carrier. | |
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MedLine Citation:
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PMID: 18442283 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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This work describes the development of polymersome-encapsulated hemoglobin (PEH) self-assembled from biodegradable and biocompatible amphiphilic diblock copolymers composed of poly(ethylene oxide) (PEO), poly(caprolactone) (PCL), and poly(lactide) (PLA). In the amphiphilic diblock, PEO functions as the hydrophilic block, while either PCL or PLA can function as the hydrophobic block. PEO, PCL, and PLA are biocompatible polymers, while the last two polymers are biodegradable. PEH dispersions were prepared by extrusion through 100 nm pore radii polycarbonate membranes. In this work, the encapsulation efficiency of human and bovine hemoglobin (hHb and bHb) in polymersomes was adjusted by varying the initial concentration of Hb. This approach yielded Hb loading capacities that were comparable to values in the literature that supported the successful resuscitation of hamsters experiencing hemorrhagic shock. Moreover, the Hb loading capacities of PEHs in this study can also be tailored simply by controlling the diblock copolymer concentration. In this study, typical Hb/diblock copolymer weight ratios ranged 1.2-1.5, with initial Hb concentrations less than 100 mg/mL. The size distribution, Hb encapsulation efficiency, oxygen affinity (P 50), cooperativity coefficient (n), and methemoglobin (metHb) level of these novel PEH dispersions were consistent with values required for efficient oxygen delivery in the systemic circulation. Taken together, our results demonstrate the development of novel PEH dispersions that are both biocompatible and biodegradable. These novel dispersions show very good promise as therapeutic oxygen carriers. |
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Authors:
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Shahid Rameez; Houssam Alosta; Andre F Palmer |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural Date: 2008-04-29 |
Journal Detail:
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Title: Bioconjugate chemistry Volume: 19 ISSN: 1520-4812 ISO Abbreviation: Bioconjug. Chem. Publication Date: 2008 May |
Date Detail:
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Created Date: 2008-05-21 Completed Date: 2008-10-06 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9010319 Medline TA: Bioconjug Chem Country: United States |
Other Details:
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Languages: eng Pagination: 1025-32 Citation Subset: IM |
Affiliation:
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Department of Chemical and Biomolecular Engineering, The Ohio State University, Columbus, Ohio 43210, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Biocompatible Materials / chemistry*, metabolism Cattle Hemoglobins / chemistry*, metabolism Humans Lactates / chemistry*, metabolism Lactones / chemistry*, metabolism Molecular Structure Molecular Weight Oxygen / chemistry*, metabolism Particle Size Polyethylene Glycols / chemistry*, metabolism |
| Grant Support | |
ID/Acronym/Agency:
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R01DK070862/DK/NIDDK NIH HHS; R01HL078840/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Biocompatible Materials; 0/Hemoglobins; 0/Lactates; 0/Lactones; 0/Polyethylene Glycols; 0/poly(ethylene oxide)-b-poly(caprolactone); 113717-54-7/polyethylene oxide-polylactic acid block copolymer; 7782-44-7/Oxygen |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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