| Biocompatibility of hydroxyapatite ceramic: response of chondrocytes in a test system using low temperature scanning electron microscopy. | |
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MedLine Citation:
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PMID: 2465329 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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An investigation is described of R37W, one of a number of porous ceramics being developed for maxillofacial surgery and the restoration of periodontal bone defects. Sensitive and precise methods are needed to assess the biocompatibility of these new materials. Mammalian chondrocytes are known to form colonies on and within porous ceramics; therefore, the tissue formed has been evaluated to gauge the response of these proliferating mesenchymal cells to the hydroxyapatite. Cell colonies, grown on R37W, have been rapidly frozen at -210 degrees C (63K) in nitrogen slush and examined by low temperature scanning electron microscopy (SEM). This method enables unfixed, fully hydrated cells to be viewed in detail and demonstrates the three-dimensional surface structure of chondrocytes in a life-like state. Features such as complex pericellular fenestrations and papilliform surface processes are believed to indicate cell viability and normality: they are not detectable by the light microscopy and SEM of fixed, dehydrated preparations. This recently recognized fine structure, together with the determination of rates of increase of cell numbers and histochemical and immunological tests of cell synthetic and secretory behaviour, provides a new guide to biocompatibility. It is concluded that the low temperature SEM of chondrocytes grown on ceramics is a valuable addition to the procedures available for the testing of hard materials before their adoption in oral surgery. |
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Authors:
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D M Lawton; M D Lamaletie; D L Gardner |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of dentistry Volume: 17 ISSN: 0300-5712 ISO Abbreviation: J Dent Publication Date: 1989 Feb |
Date Detail:
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Created Date: 1989-04-04 Completed Date: 1989-04-04 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 0354422 Medline TA: J Dent Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 21-7 Citation Subset: D; IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Biocompatible Materials* Cartilage / cytology Cell Count Cells, Cultured Ceramics* Cold Temperature Dogs Hyalin / cytology Hydroxyapatites* Male Microscopy, Electron, Scanning Staining and Labeling Surface Properties |
| Chemical | |
Reg. No./Substance:
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0/Biocompatible Materials; 0/Ceramics; 0/Hydroxyapatites |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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