Document Detail


Biocompatibility of a glucose-free, acidic lactated solution for peritoneal dialysis evaluated by population analysis of mesothelium.
MedLine Citation:
PMID:  9678434     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Glucose-enriched, racemic lactate buffered solutions for peritoneal dialysis induce a significant reduction of cell viability as well as a hypertrophic, senescent phenotype of the exposed monolayer. The present study was designed to verify whether the aforementioned changes resulted form the buffer, from the osmotic agent, or from a combined effect of both. Mice were acutely (2 h) and long-term (15 and 30 days) exposed to daily intraperitoneal injections of a racemic lactate, heat-sterilized, low-pH (5.2), glucose-free solution. Imprints of the monolayer were taken at the end of each time interval. The glucose-free lactated buffer used in the present study did not induce significant changes in density distribution, mean cell size, mean cytoplasmic surface area, prevalence of large cells, multinucleation, proportion of observed cells in mitosis, and cell viability. So far, the previously mentioned hypertrophic phenotype appears to derive from substantial alterations in the cell cycle of mesothelium exposed to high concentrations of glucose and/or advanced glycosylation end products and unrelated to lactate.
Authors:
V Wajsbrot; A Shostak; L Gotloib; R Kushnier
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Nephron     Volume:  79     ISSN:  0028-2766     ISO Abbreviation:  Nephron     Publication Date:  1998  
Date Detail:
Created Date:  1998-09-21     Completed Date:  1998-09-21     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  0331777     Medline TA:  Nephron     Country:  SWITZERLAND    
Other Details:
Languages:  eng     Pagination:  322-32     Citation Subset:  IM    
Affiliation:
Department of Nephrology and Hypertension, 'Ha'Emek' Medical Center, Afula, Israel.
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MeSH Terms
Descriptor/Qualifier:
Acids / pharmacology*
Animals
Buffers
Cell Nucleus / drug effects
Cell Survival / drug effects
Dialysis Solutions / chemistry*,  pharmacology
Epithelial Cells / cytology,  drug effects
Epithelium / drug effects
Glucose / pharmacology*
Hydrogen-Ion Concentration
Lactates / pharmacology*
Materials Testing
Mice
Mice, Inbred Strains
Mitosis / drug effects
Peritoneal Dialysis / methods*
Chemical
Reg. No./Substance:
0/Acids; 0/Buffers; 0/Dialysis Solutions; 0/Lactates; 50-99-7/Glucose

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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