| Biocompatibility of contact lens solutions using confocal laser scanning microscopy and the in vitro bovine cornea. | |
| | |
MedLine Citation:
|
PMID: 17993827 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
PURPOSE: This study used high-resolution research confocal laser scanning microscopy to study the epithelium of fresh bovine corneas treated with two contact lens solutions. METHODS: Fresh bovine corneal buttons were stained with a combination of the cell-permeable mitochondrial- and nuclear-specific dyes rhodamine 123 and Hoechst 33342 or acridine orange and imaged with a confocal laser scanning microscope. High-resolution X,Y images of the corneal epithelium were taken with a Zeiss 510 confocal laser scanning microscope at a 0.44-062 -microm Z step, and three-dimensional images of the entire thickness of the corneal epithelium were reconstructed. Semiautomated quantitative MatLab software analysis was applied to count the mitochondria in each optical slice. The effects of treatment for 30 minutes with OPTI-FREE Express and ReNu MultiPlus No Rub were investigated and compared to Hanks' balanced salt solution controls. RESULTS: This study showed the feasibility of using the confocal laser scanning microscope with fluorescent dyes to study organelles throughout the epithelial layers of fresh unfixed bovine corneas. The mitochondria in the intermediate and basal epithelium are abundant and range in length from less than 1 microm to threads greater than 15 microm, with a tendency to accumulate around the nuclei. Image analysis indicated significantly fewer mitochondria only in the superficial epithelium after treatment with ReNu MultiPlus No Rub treatment. OPTI-FREE Express treatment produced significantly fewer mitochondria not only in the superficial but also in the intermediate epithelial layers. CONCLUSIONS: OPTI-FREE Express has the potential for deeper mitochondrial effects compared to ReNu MultiPlus No Rub and Hanks' balanced salt solution controls. |
| | |
Authors:
|
Vladimir Bantseev; David J McCanna; Jean-Yves Driot; Keith W Ward; Jacob G Sivak |
Related Documents
:
|
6605977 - Quantized nature of sarcomere shortening steps. 20333127 - Images of phase edges in conventional and scanning optical microscopes. 16406937 - Novel virtual biopsy of the kidney with near infrared, reflectance confocal microscopy:... 15566497 - An optical-axis freezing stage for laser-scanning microscopy of broad ice-water interfa... 19439627 - Use of thermography to screen for subclinical bumblefoot in poultry. 20560027 - Intramyocardial navigation and mapping for stem cell delivery. |
Publication Detail:
|
Type: In Vitro; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Eye & contact lens Volume: 33 ISSN: 1542-2321 ISO Abbreviation: Eye Contact Lens Publication Date: 2007 Nov |
Date Detail:
|
Created Date: 2007-11-12 Completed Date: 2008-01-08 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 101160941 Medline TA: Eye Contact Lens Country: United States |
Other Details:
|
Languages: eng Pagination: 308-16 Citation Subset: IM |
Affiliation:
|
School of Optometry, University of Waterloo, 200 University Avenue West, Waterloo, Ontario, Canada. vbantsee@uwaterloo.ca |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Acridine Orange
/
diagnostic use Animals Benzimidazoles / diagnostic use Cattle Contact Lens Solutions / pharmacology* Cornea / drug effects*, ultrastructure Epithelium, Corneal / drug effects, ultrastructure Feasibility Studies Fluorescent Dyes / diagnostic use Image Processing, Computer-Assisted Imaging, Three-Dimensional Materials Testing / methods* Microscopy, Confocal* / methods Mitochondria / drug effects, ultrastructure Organelles / drug effects, ultrastructure Rhodamine 123 / diagnostic use |
| Chemical | |
Reg. No./Substance:
|
0/Benzimidazoles; 0/Contact Lens Solutions; 0/Fluorescent Dyes; 23491-52-3/HOE 33342; 62669-70-9/Rhodamine 123; 65-61-2/Acridine Orange |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Rigid gas-permeable contact lenses and severe higher-order aberrations in postsurgical corneas.
Next Document: Benefits of contact lens wear for children and teens.