Document Detail


Biocompatibility of contact lens solutions using confocal laser scanning microscopy and the in vitro bovine cornea.
MedLine Citation:
PMID:  17993827     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
PURPOSE: This study used high-resolution research confocal laser scanning microscopy to study the epithelium of fresh bovine corneas treated with two contact lens solutions. METHODS: Fresh bovine corneal buttons were stained with a combination of the cell-permeable mitochondrial- and nuclear-specific dyes rhodamine 123 and Hoechst 33342 or acridine orange and imaged with a confocal laser scanning microscope. High-resolution X,Y images of the corneal epithelium were taken with a Zeiss 510 confocal laser scanning microscope at a 0.44-062 -microm Z step, and three-dimensional images of the entire thickness of the corneal epithelium were reconstructed. Semiautomated quantitative MatLab software analysis was applied to count the mitochondria in each optical slice. The effects of treatment for 30 minutes with OPTI-FREE Express and ReNu MultiPlus No Rub were investigated and compared to Hanks' balanced salt solution controls. RESULTS: This study showed the feasibility of using the confocal laser scanning microscope with fluorescent dyes to study organelles throughout the epithelial layers of fresh unfixed bovine corneas. The mitochondria in the intermediate and basal epithelium are abundant and range in length from less than 1 microm to threads greater than 15 microm, with a tendency to accumulate around the nuclei. Image analysis indicated significantly fewer mitochondria only in the superficial epithelium after treatment with ReNu MultiPlus No Rub treatment. OPTI-FREE Express treatment produced significantly fewer mitochondria not only in the superficial but also in the intermediate epithelial layers. CONCLUSIONS: OPTI-FREE Express has the potential for deeper mitochondrial effects compared to ReNu MultiPlus No Rub and Hanks' balanced salt solution controls.
Authors:
Vladimir Bantseev; David J McCanna; Jean-Yves Driot; Keith W Ward; Jacob G Sivak
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Eye & contact lens     Volume:  33     ISSN:  1542-2321     ISO Abbreviation:  Eye Contact Lens     Publication Date:  2007 Nov 
Date Detail:
Created Date:  2007-11-12     Completed Date:  2008-01-08     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101160941     Medline TA:  Eye Contact Lens     Country:  United States    
Other Details:
Languages:  eng     Pagination:  308-16     Citation Subset:  IM    
Affiliation:
School of Optometry, University of Waterloo, 200 University Avenue West, Waterloo, Ontario, Canada. vbantsee@uwaterloo.ca
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MeSH Terms
Descriptor/Qualifier:
Acridine Orange / diagnostic use
Animals
Benzimidazoles / diagnostic use
Cattle
Contact Lens Solutions / pharmacology*
Cornea / drug effects*,  ultrastructure
Epithelium, Corneal / drug effects,  ultrastructure
Feasibility Studies
Fluorescent Dyes / diagnostic use
Image Processing, Computer-Assisted
Imaging, Three-Dimensional
Materials Testing / methods*
Microscopy, Confocal* / methods
Mitochondria / drug effects,  ultrastructure
Organelles / drug effects,  ultrastructure
Rhodamine 123 / diagnostic use
Chemical
Reg. No./Substance:
0/Benzimidazoles; 0/Contact Lens Solutions; 0/Fluorescent Dyes; 23491-52-3/HOE 33342; 62669-70-9/Rhodamine 123; 65-61-2/Acridine Orange

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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