Document Detail

Biocompatibility of bioresorbable poly(L-lactic acid) composite scaffolds obtained by supercritical gas foaming with human fetal bone cells.
MedLine Citation:
PMID:  16411809     Owner:  NLM     Status:  MEDLINE    
The aim of this investigation was to test the biocompatibility of three-dimensional bioresorbable foams made of poly(L-lactic acid) (PLA), alone or filled with hydroxyapatite (HA) or beta-tricalcium phosphate (beta-TCP), with human primary osteoblasts, using a direct contact method. Porous constructs were processed by supercritical gas foaming, after a melt-extrusion of ceramic/polymer mixture. Three neat polymer foams, with pore sizes of 170, 310, and 600 microm, and two composite foams, PLA/5 wt% HA and PLA/5 wt% beta-TCP, were examined over a 4-week culture period. The targeted application is the bone tissue-engineering field. For this purpose, human fetal and adult bone cells were chosen because of their highly osteogenic potential. The association of fetal bone cells and composite scaffold should lead to in vitro bone formation. The polymer and composite foams supported adhesion and intense proliferation of seeded cells, as revealed by scanning electron microscopy. Cell differentiation toward osteoblasts was demonstrated by alkaline phosphatase (ALP) enzymatic activity, gamma-carboxylated Gla-osteocalcin production, and the onset of mineralization. The addition of HA or beta-TCP resulted in higher ALP enzymatic activity for fetal bone cells and a stronger production of Gla-osteocalcin for adult bone cells.
Marc-Olivier Montjovent; Laurence Mathieu; Boris Hinz; Lee Laurent Applegate; Pierre-Etienne Bourban; Pierre-Yves Zambelli; Jan-Anders Månson; Dominique P Pioletti
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Tissue engineering     Volume:  11     ISSN:  1076-3279     ISO Abbreviation:  Tissue Eng.     Publication Date:    2005 Nov-Dec
Date Detail:
Created Date:  2006-01-17     Completed Date:  2006-04-28     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9505538     Medline TA:  Tissue Eng     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1640-9     Citation Subset:  IM    
Laboratoire de Recherche en Orthopédie, Ecole Polytechnique Fédérale de Lausanne, Lausanne, Switzerland.
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MeSH Terms
Bone Substitutes*
Calcium Phosphates*
Cells, Cultured
Fetus / physiology*,  ultrastructure
Lactic Acid*
Materials Testing / methods
Osteoblasts / physiology*,  ultrastructure
Osteogenesis / physiology*
Tissue Engineering / methods
Reg. No./Substance:
0/Bone Substitutes; 0/Calcium Phosphates; 0/Polymers; 0/beta-tricalcium phosphate; 26100-51-6/poly(lactic acid); 50-21-5/Lactic Acid

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