| Biochemistry of radioiodinated free fatty acids. | |
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MedLine Citation:
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PMID: 2779539 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Radioiodinated free fatty acids have been developed to study myocardial metabolism non-invasively in man. In the present study the distribution of radiolabeled lipids in the myocardium and in arterial and coronary sinus blood was evaluated following injection of three commonly used iodinated fatty acids in fasted (n = 5) and lactate loaded (n = 3) dogs. Five minutes after simultaneous i.v. injection of radioiodinated 17-I-heptadecanoic acid (IHDA), 15-(p-I-phenyl) pentadecanoic acid (IPPA) and 15-(p-I-phenyl)-3,3-dimethyl-pentadecanoic acid (DMIPPA) a biopsy specimen and samples of arterial and coronary sinus blood were taken. After extraction and TLC the relative distribution of radioactivity in the aqueous phase (containing the oxidation products), pellet and organic phase was calculated. The organic phase was further divided into phospholipids, diglycerides, free fatty acids, triglycerides and cholesterol-esters. Seventy two percent of IHDA was oxidized, 36% of IPPA and 7% of DMIPPA. The organic phase consisted primarily of triglycerides and phospholipids. The ratios of triglycerides to phospholipids were about the same for IHDA, IPPA and DMIPPA (0.58, 0.65 and 0.50, respectively). Free IHDA in tissue samples was low (4%) and elevated for IPPA and DMIPPA, (17% and 37%). During lactate loading triglycerides were higher for all three fatty acids. For IHDA and IPPA this increase was paralleled by a decrease in the aqueous phase, in case of DMIPPA the aqueous phase remained the same. Five minutes after injection most of the organic phase of both arterial and coronary sinus blood consisted of the injected fatty acids, the aqueous phase contained oxidation products. There were only minor differences during lactate loading.(ABSTRACT TRUNCATED AT 250 WORDS) |
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Authors:
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F C Visser; C M Duwel; M J van Eenige; J P Roos; F F Knapp; G J van der Vusse |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Molecular and cellular biochemistry Volume: 88 ISSN: 0300-8177 ISO Abbreviation: Mol. Cell. Biochem. Publication Date: 1989 Jun 27-Jul 24 |
Date Detail:
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Created Date: 1989-10-26 Completed Date: 1989-10-26 Revised Date: 2003-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0364456 Medline TA: Mol Cell Biochem Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 185-90 Citation Subset: IM |
Affiliation:
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Dept of Cardiology, Free University Hospital, Amsterdam, The Netherlands. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Dogs Fatty Acids / pharmacokinetics* Iodine Radioisotopes / diagnostic use Iodobenzenes / pharmacokinetics* Lactates / pharmacology Myocardium / metabolism* Tissue Distribution |
| Chemical | |
Reg. No./Substance:
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0/Fatty Acids; 0/Iodine Radioisotopes; 0/Iodobenzenes; 0/Lactates; 104426-56-4/15-(4-iodophenyl)-3,3-dimethylpentadecanoic acid; 63556-27-4/17-iodoheptadecanoic acid; 80479-93-2/omega-(4-iodophenyl)pentadecanoic acid |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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