Document Detail


Biochemistry of L-proline-triggered germination of Bacillus megaterium spores.
MedLine Citation:
PMID:  35526     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The mechanism by which L-proline triggers germination in Bacillus megaterium QM B1551 spores was investigated. First, brief exposure of spores to L-proline, followed by dilution, was sufficient to trigger germination. Once germination was triggered, the spores continued initiation of germination and did not require high concentrations of L-proline. Triggering of germination was pH and temperature dependent. Second, enzymes for L-proline catabolism were absent in spores, and several non-metabolizable analogs of L-proline were effective trigger compounds. Third, triggering of germination occurred in the presence of inhibitors of proton motive force production, oxygen uptake, and metabolism. Fourth, uptake of L-proline occurred after the triggering of germination. These results argue that neither uptake nor metabolism of L-proline was necessary to trigger germination. Instead, L-proline probably causes a biophysical alteration in the spores that triggers the biochemical changes in germination.
Authors:
D P Rossignol; J C Vary
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of bacteriology     Volume:  138     ISSN:  0021-9193     ISO Abbreviation:  J. Bacteriol.     Publication Date:  1979 May 
Date Detail:
Created Date:  1979-07-28     Completed Date:  1979-07-28     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  2985120R     Medline TA:  J Bacteriol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  431-41     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Bacillus megaterium / drug effects*,  growth & development,  metabolism
Hydrogen-Ion Concentration
Oxygen Consumption
Proline / analogs & derivatives,  metabolism,  pharmacology*
Spores, Bacterial / drug effects,  growth & development
Stereoisomerism
Temperature
Chemical
Reg. No./Substance:
147-85-3/Proline
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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