Document Detail


Biochemical studies of embryogenic and non-embryogenic callus of Cardiospermum halicacabum L.
MedLine Citation:
PMID:  15991583     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Some physiological and biochemical changes were measured between embryogenic and non-embryogenic callus obtained from Cardiospermum halicacabum. Combination of auxin with cytokinin was more favourable for high amount of callus formation. 2,4-D played a key role in triggering somatic embryo formation. Embryogenic callus had more total carbohydrate and starch contents, total free amino acids, nucleic acids, phenols and ascorbic acid. Non-embryogenic callus exhibited high chlorophyll content, total soluble sugar, protein, ammonia and enzymes like peroxidase and polyphenol oxidase. Thus, the present study indicated that the process of somatic embryogenesis was characterized by some biochemical and physiological changes induced by plant growth regulators.
Authors:
M Jeyaseelan; M V Rao
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Indian journal of experimental biology     Volume:  43     ISSN:  0019-5189     ISO Abbreviation:  Indian J. Exp. Biol.     Publication Date:  2005 Jun 
Date Detail:
Created Date:  2005-07-04     Completed Date:  2005-08-19     Revised Date:  2011-01-02    
Medline Journal Info:
Nlm Unique ID:  0233411     Medline TA:  Indian J Exp Biol     Country:  India    
Other Details:
Languages:  eng     Pagination:  555-60     Citation Subset:  IM    
Affiliation:
Department of Plant Science, Bharathidasan University, Tiruchirappalli 620 024, India.
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MeSH Terms
Descriptor/Qualifier:
Carbohydrates / chemistry
Catechol Oxidase / chemistry
Cells, Cultured
Chlorophyll / chemistry,  metabolism
Culture Techniques
Cytokinins / chemistry*
Dose-Response Relationship, Drug
Indoleacetic Acids / chemistry*
Peroxidases / metabolism
Plant Growth Regulators / physiology
Plants / metabolism
Sapindaceae / embryology*,  metabolism*
Chemical
Reg. No./Substance:
0/Carbohydrates; 0/Cytokinins; 0/Indoleacetic Acids; 0/Plant Growth Regulators; 1406-65-1/Chlorophyll; EC 1.10.3.1/Catechol Oxidase; EC 1.11.1.-/Peroxidases

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