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Biochemical studies on cell fusion. I. Lipid composition of fusion-resistant cells.
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MedLine Citation:
PMID:  4044645     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A series of stable cell mutants of mouse fibroblasts were previously isolated (Roos, D. S. and R. L. Davidson, 1980, Somatic Cell Genet., 6:381-390) that exhibit varying degrees of resistance to the fusion-inducing effect of polyethylene glycol (PEG), but are morphologically similar to the parental cells from which they were derived. Biochemical analysis of these mutant cell lines has revealed differences in whole cell lipid composition which are directly correlated with their susceptibility to fusion. Fusion-resistant cells contain elevated levels of neutral lipids, particularly triglycerides and an unusual ether-linked lipid, O-alkyl, diacylglycerol. This ether lipid is increased approximately 35-fold over parental cells in the most highly PEG-resistant cell line. Fusion-resistant cells also contain more highly saturated fatty acyl chains (ratio of saturated to polyunsaturated fatty acids [S/P ratio] approximately 4:1) than the parental line (S/P ratio approximately 1:1). Cells which are intermediate in their resistance to PEG have ether lipid and fatty acid composition which is intermediate between the parental cells and the most fusion-resistant mutants. In a related communication (Roos, D. S. and P. W. Choppin, 1985, J. Cell. Biol., 100:1591-1598) evidence is presented that alteration of lipid content can predictably control the fusion response of these cells.
Authors:
D S Roos; P W Choppin
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The Journal of cell biology     Volume:  101     ISSN:  0021-9525     ISO Abbreviation:  J. Cell Biol.     Publication Date:  1985 Oct 
Date Detail:
Created Date:  1985-11-07     Completed Date:  1985-11-07     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0375356     Medline TA:  J Cell Biol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1578-90     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Fusion* / drug effects
Chromatography, Thin Layer
Diglycerides / analysis
Fatty Acids / analysis
L Cells (Cell Line) / analysis,  drug effects,  physiology*
Lipids / analysis*,  classification
Mice
Phospholipids / analysis
Plasmalogens / analysis
Polyethylene Glycols / pharmacology
Grant Support
ID/Acronym/Agency:
AI-05600/AI/NIAID NIH HHS; AI-07233/AI/NIAID NIH HHS; CA-18213/CA/NCI NIH HHS
Chemical
Reg. No./Substance:
0/Diglycerides; 0/Fatty Acids; 0/Lipids; 0/Phospholipids; 0/Plasmalogens; 0/Polyethylene Glycols
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Full Text
Journal Information
Journal ID (nlm-ta): J Cell Biol
ISSN: 0021-9525
ISSN: 1540-8140
Publisher: The Rockefeller University Press
Article Information
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Print publication date: Day: 1 Month: 10 Year: 1985
Volume: 101 Issue: 4
First Page: 1578 Last Page: 1590
ID: 2113923
Publisher Id: 86008479
PubMed Id: 4044645

Biochemical studies on cell fusion. I. Lipid composition of fusion- resistant cells


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