Document Detail

Biochemical and structural changes in cultured heart cells induced by metabolic inhibition.
MedLine Citation:
PMID:  3688218     Owner:  NLM     Status:  MEDLINE    
We examined the relationship between ionic homeostasis, ATP, and irreversible cell injury in cultured embryonic chick heart cells treated with rotenone (10(-4) M) alone or in combination with iodoacetate (IAA) (10(-3) M), in the presence of extracellular calcium (Ca0) (2.7 mM) and its nominal absence. Changes in Na, K, and total cell Ca content did not correlate with parameters indicative of irreversible injury, i.e., ultrastructural damage or lactate dehydrogenase (LDH) release. Because structural defects in the plasma membrane occurred without a significant release of LDH after exposure to rotenone plus IAA for 1 h, LDH release appears to be a relatively late event in cell injury. In addition, cells exposed to rotenone in the presence of Ca0 for 2.5 h showed a significant fall in ATP and a rise in LDH release. This response was attenuated in the nominal absence of Ca0, and the addition of rotenone caused an eightfold increase in intracellular sodium (Nai), whereas in the presence of Ca0, Nai increased only threefold in 2.5 h. Thus Ca0 appears to promote Nai-Ca0 exchange and lead to an increase in cell Ca that can then stimulate ATP breakdown by Ca-activated ATPases. Of the measured variables associated with myocardial cell injury, a decline in ATP correlates best with changes in either LDH or morphology. The apparent lack of correlation between changes in intracellular ion content, LDH release, and morphology supports the conclusion that myocardial cell injury is a multifactorial process.
E Murphy; A LeFurgey; M Lieberman
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The American journal of physiology     Volume:  253     ISSN:  0002-9513     ISO Abbreviation:  Am. J. Physiol.     Publication Date:  1987 Nov 
Date Detail:
Created Date:  1988-01-06     Completed Date:  1988-01-06     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0370511     Medline TA:  Am J Physiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  C700-6     Citation Subset:  IM    
Department of Physiology, Duke University Medical Center, Durham 27710.
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MeSH Terms
Adenosine Triphosphate / metabolism
Calcium / metabolism
Cells, Cultured
Chick Embryo
Glycolysis / drug effects
Heart / drug effects
Iodoacetates / pharmacology
Iodoacetic Acid
L-Lactate Dehydrogenase / metabolism
Microscopy, Electron
Myocardium* / metabolism,  ultrastructure
Oxidative Phosphorylation / drug effects
Potassium / metabolism
Rotenone / pharmacology*
Sodium / metabolism
Grant Support
Reg. No./Substance:
0/Iodoacetates; 56-65-5/Adenosine Triphosphate; 64-69-7/Iodoacetic Acid; 7440-09-7/Potassium; 7440-23-5/Sodium; 7440-70-2/Calcium; 83-79-4/Rotenone; EC Dehydrogenase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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