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Biochemical properties of a novel glycoside hydrolase family 1 β-glucosidase (PtBglu1) from Paecilomyces thermophila expressed in Pichia pastoris.
MedLine Citation:
PMID:  23218368     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
A novel β-glucosidase gene (PtBglu1) from the thermophilic fungus, Paecilomyces thermophila, was cloned and expressed in Pichia pastoris. PtBglu1 contained an open reading frame of 1440-bp nucleotides and encoded a protein of 479 amino acids which showed significant similarity to other fungal β-glucosidases from glycoside hydrolase (GH) family 1. The recombinant β-glucosidase (PtBglu1) was secreted at high level of 190.2UmL(-1) in high cell density fermentor (5L). PtBglu1 was purified to homogeneity, and was found to be a glycoprotein with molecular mass of 56.7kDa. The purified PtBglu1 showed optimum catalytic activity at pH 6.0 and 55°C. The enzyme exhibited broad substrate specificity with highest activity toward pNP-β-d-glucopyranoside, followed by pNP-β-d-galactopyranoside and cellobiose. The K(m) values for pNP-β-d-glucopyranoside, cellobiose, gentiobiose and salicin were 0.55mM, 1.0mM, 1.74mM and 6.85mM, respectively. These properties make PtBglu1 a potential candidate for various industrial applications.
Authors:
Shaoqing Yang; Chengwei Hua; Qiaojuan Yan; Yinan Li; Zhengqiang Jiang
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Publication Detail:
Type:  Journal Article     Date:  2012-10-08
Journal Detail:
Title:  Carbohydrate polymers     Volume:  92     ISSN:  1879-1344     ISO Abbreviation:  Carbohydr Polym     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2012-12-10     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8307156     Medline TA:  Carbohydr Polym     Country:  England    
Other Details:
Languages:  eng     Pagination:  784-91     Citation Subset:  IM    
Copyright Information:
Copyright © 2012 Elsevier Ltd. All rights reserved.
Affiliation:
Department of Biotechnology, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China.
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