| Biochemical properties of canine serum ferritin: iron content and nonbinding to concanavalin A. | |
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MedLine Citation:
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PMID: 11247038 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A sandwich enzyme-linked immunosorbent assay using H-subunit-rich canine heart ferritin as a standard has been developed for measuring canine serum ferritin which is H-subunit-rich. Serum ferritin concentrations in 51 normal dogs ranged from 143 to 1766 ng ml(-1), with a mean value of 479 +/- 286 (SD) ng ml(-1). Serum ferritin iron concentrations as determined by an immunoprecipitation technique ranged from 30.4 to 115.9 ng ml(-1) in 15 normal dogs with serum ferritin protein levels of 298 to 959 ng ml(-1). There was a significant linear correlation between the serum ferritin iron and protein levels (r=0.9441, P<0.001), and the mean iron/protein ratio of serum ferritin was 0.112 +/- 0.017. When canine sera were incubated with concanavalin A-Sepharose 4B, we observed the apparent binding of serum ferritin to concanavalin A. However, ferritin obtained by heat-treating the sera at pH 4.8 to remove the ferritin-binding proteins did not bind to the lectin. These results suggest that canine serum ferritin contains a considerable amount of iron but no concanavalin A-binding G subunit present in human serum ferritin. |
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Authors:
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K Watanabe; N Muranishi; Y Murata; K Orino; S Okano; S Yamamoto |
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Publication Detail:
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Type: In Vitro; Journal Article |
Journal Detail:
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Title: Biometals : an international journal on the role of metal ions in biology, biochemistry, and medicine Volume: 13 ISSN: 0966-0844 ISO Abbreviation: Biometals Publication Date: 2000 Dec |
Date Detail:
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Created Date: 2001-03-14 Completed Date: 2001-04-05 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9208478 Medline TA: Biometals Country: Netherlands |
Other Details:
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Languages: eng Pagination: 319-24 Citation Subset: IM |
Affiliation:
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Laboratory of Biochemistry, School of Veterinary Medicine and Animal Sciences, Kitasato University, Towada, Aomori, Japan. watanabe@vmas.kitasato-u.ac.jp |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Concanavalin A / metabolism Dogs Enzyme-Linked Immunosorbent Assay / methods, standards Ferritins / blood*, chemistry*, immunology Immunochemistry Iron / analysis, blood Liver / chemistry Myocardium / chemistry Protein Binding Protein Subunits Reference Standards |
| Chemical | |
Reg. No./Substance:
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0/Protein Subunits; 11028-71-0/Concanavalin A; 7439-89-6/Iron; 9007-73-2/Ferritins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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