Document Detail


Biochemical properties of canine serum ferritin: iron content and nonbinding to concanavalin A.
MedLine Citation:
PMID:  11247038     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A sandwich enzyme-linked immunosorbent assay using H-subunit-rich canine heart ferritin as a standard has been developed for measuring canine serum ferritin which is H-subunit-rich. Serum ferritin concentrations in 51 normal dogs ranged from 143 to 1766 ng ml(-1), with a mean value of 479 +/- 286 (SD) ng ml(-1). Serum ferritin iron concentrations as determined by an immunoprecipitation technique ranged from 30.4 to 115.9 ng ml(-1) in 15 normal dogs with serum ferritin protein levels of 298 to 959 ng ml(-1). There was a significant linear correlation between the serum ferritin iron and protein levels (r=0.9441, P<0.001), and the mean iron/protein ratio of serum ferritin was 0.112 +/- 0.017. When canine sera were incubated with concanavalin A-Sepharose 4B, we observed the apparent binding of serum ferritin to concanavalin A. However, ferritin obtained by heat-treating the sera at pH 4.8 to remove the ferritin-binding proteins did not bind to the lectin. These results suggest that canine serum ferritin contains a considerable amount of iron but no concanavalin A-binding G subunit present in human serum ferritin.
Authors:
K Watanabe; N Muranishi; Y Murata; K Orino; S Okano; S Yamamoto
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Publication Detail:
Type:  In Vitro; Journal Article    
Journal Detail:
Title:  Biometals : an international journal on the role of metal ions in biology, biochemistry, and medicine     Volume:  13     ISSN:  0966-0844     ISO Abbreviation:  Biometals     Publication Date:  2000 Dec 
Date Detail:
Created Date:  2001-03-14     Completed Date:  2001-04-05     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9208478     Medline TA:  Biometals     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  319-24     Citation Subset:  IM    
Affiliation:
Laboratory of Biochemistry, School of Veterinary Medicine and Animal Sciences, Kitasato University, Towada, Aomori, Japan. watanabe@vmas.kitasato-u.ac.jp
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MeSH Terms
Descriptor/Qualifier:
Animals
Concanavalin A / metabolism
Dogs
Enzyme-Linked Immunosorbent Assay / methods,  standards
Ferritins / blood*,  chemistry*,  immunology
Immunochemistry
Iron / analysis,  blood
Liver / chemistry
Myocardium / chemistry
Protein Binding
Protein Subunits
Reference Standards
Chemical
Reg. No./Substance:
0/Protein Subunits; 11028-71-0/Concanavalin A; 7439-89-6/Iron; 9007-73-2/Ferritins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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