Document Detail

Biochemical outcome of blocking the ergot alkaloid pathway of a grass endophyte.
MedLine Citation:
PMID:  14558758     Owner:  NLM     Status:  MEDLINE    
Neotyphodium sp. Lp1, an endophytic fungus from perennial ryegrass (Lolium perenne), produces the mycotoxin ergovaline in infected grasses, whereas a mutant in which a particular peptide synthetase gene is knocked out does not. We examined the impact of this knockout on other constituents of the ergot alkaloid pathway. Two simple lysergic acid amides, ergine and a previously undescribed amide, were eliminated by the knockout. Lysergic acid accumulated in the knockout endophyte, but quantities were only 13% of the total lysergic acid derivatives accumulated in the wild type. Concentrations of several clavines were not substantially affected. However, a novel clavine accumulated to higher concentrations in perennial ryegrass containing the knockout strain. The results indicate that production of simple lysergic acid amides requires the activity or products of the ergovaline-associated peptide synthetase and that the regulation of ergot alkaloid production is modified in response to the relatively late block in the pathway.
Daniel G Panaccione; Brian A Tapper; Geoffrey A Lane; Elizabeth Davies; Karl Fraser
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Journal of agricultural and food chemistry     Volume:  51     ISSN:  0021-8561     ISO Abbreviation:  J. Agric. Food Chem.     Publication Date:  2003 Oct 
Date Detail:
Created Date:  2003-10-15     Completed Date:  2003-12-11     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0374755     Medline TA:  J Agric Food Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  6429-37     Citation Subset:  IM    
Division of Plant & Soil Sciences, 401 Brooks Hall, P.O. Box 6058, West Virginia University, Morgantown, WV 26506-6058, USA.
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MeSH Terms
Ascomycota / enzymology,  genetics*,  metabolism*
Ergot Alkaloids / analysis,  chemistry,  metabolism*
Lolium / microbiology*
Lysergic Acid / metabolism
Peptide Synthases / genetics,  metabolism
Reg. No./Substance:
0/Ergot Alkaloids; 82-58-6/Lysergic Acid; EC 6.3.2.-/Peptide Synthases; EC 6.3.2.-/lysergyl peptide synthetase

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