Document Detail


Biochemical and morphological study of adriamycin-induced changes in murine neuroblastoma cells.
MedLine Citation:
PMID:  7078939     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Cholinergic murine neuroblastoma cells, maintained in vitro, were exposed to a low concentration (0.4 micrograms/ml) of adriamycin. Morphologically the treated cells appeared to differentiate. The cell bodies increased in size from an average fixed cell body diameter of 7-13 to 25-40 micrometers, the cells developed long processes, became argyrophilic and the percentage of cells undergoing mitosis decreased relative to controls. Acetylcholine esterase activity increased in the drug-treated cells suggesting induction of differentiation. However, choline acetyltransferase activity and ganglioside composition remained unchanged. In addition, inoculation of mice with 2 x 10(5) viable drug-treated or control cells resulted in all of the mice developing neuroblastoma. No differences were observed in either the rate of tumor development or survival times. These results suggest that neuroblastoma cells may survive adriamycin treatment by becoming 'differentiated', ceasing cell division until conditions favor their undergoing another cell cycle.
Authors:
C L Schengrund; B A Sheffler
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Oncology     Volume:  39     ISSN:  0030-2414     ISO Abbreviation:  Oncology     Publication Date:  1982  
Date Detail:
Created Date:  1982-07-22     Completed Date:  1982-07-22     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  0135054     Medline TA:  Oncology     Country:  SWITZERLAND    
Other Details:
Languages:  eng     Pagination:  185-90     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Acetylcholinesterase / metabolism
Animals
Cell Differentiation / drug effects*
Cell Line
Cells, Cultured
Choline O-Acetyltransferase / metabolism
Doxorubicin / pharmacology*
Drug Evaluation, Preclinical
Gangliosides / metabolism
Mice
Mitotic Index
Neoplasms, Experimental / drug therapy
Neuroblastoma / drug therapy*,  metabolism,  pathology
Chemical
Reg. No./Substance:
0/Gangliosides; 23214-92-8/Doxorubicin; EC 2.3.1.6/Choline O-Acetyltransferase; EC 3.1.1.7/Acetylcholinesterase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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