| Biochemical and morphologic effects after extended liver resection in rats: preliminary results. | |
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MedLine Citation:
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PMID: 20534224 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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After hepatic resection and transplantation with a partial graft, death and regeneration of the hepatocytes coexist in the liver. However, when the functional liver mass is inadequate to ensure a proper balance between regeneration vs functional and metabolic demands, small-for-size syndrome develops. We assessed the early effects of extended hepatic resection on liver function in a rat model. Six male Sprague-Dawley rats underwent 80% resection of the liver, and 6 rats served as a control group. At 6 hours after resection, blood samples were obtained from the hepatic vein for measurement of reduced glutathione (GSH), oxidized glutathione (GSSG), and hepatic venous oxygen saturation (Shvo(2)), and for standard liver function tests including determination of concentrations of alanine aminotransferase (ALT), aspartate aminotransferase (AST), gamma-glutamyl transpeptidase, and total bilirubin. The remnant lobe was removed for GSH assay and histopathologic analysis. In the resection group, values were significantly higher for ALT (P = .002), AST (P = .002), and Shvo(2) (P = .01), whereas a significant decrease was observed for blood GSH (P = .009) but not liver GSH. Also in the resection group, we observed characteristic hepatocyte vacuolization with a gradient from periportal acinar zone 1 to the centrolobular area, the presence of hemorrhagic necrosis, and several leukocyte adhesions. The Shvo(2) and GSH data suggest early alteration of oxygen metabolism, as demonstrated by the reduction in oxygen uptake and decreased liver GSH secretion, with preservation of hepatic GSH. Mitochondrial dysfunction and oxidative injury seem to have a crucial role in early onset of liver damage. |
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Authors:
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S Di Domenico; G Santori; E Balbis; N Traverso; R Gentile; B Bocca; M Gelli; E Andorno; D Cottalasso; U Valente |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Transplantation proceedings Volume: 42 ISSN: 1873-2623 ISO Abbreviation: Transplant. Proc. Publication Date: 2010 May |
Date Detail:
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Created Date: 2010-06-10 Completed Date: 2010-10-29 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0243532 Medline TA: Transplant Proc Country: United States |
Other Details:
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Languages: eng Pagination: 1061-5 Citation Subset: IM |
Copyright Information:
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Copyright (c) 2010. Published by Elsevier Inc. |
Affiliation:
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Department of Transplantation, San Martino University Hospital, Genoa, Italy. didomenico.stefano@gmail.com |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Alanine Transaminase
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blood Animals Anticonvulsants / pharmacology Aspartate Aminotransferases / blood GABA Modulators / pharmacology Hepatectomy Hepatocytes / cytology, physiology Liver / anatomy & histology, physiology Liver Function Tests Liver Regeneration / physiology* Liver Transplantation / physiology* Male Mitochondria, Liver / pathology, physiology Organ Size Portal System / physiology Rats Rats, Sprague-Dawley Tiletamine / pharmacology Vena Cava, Inferior / surgery Zolazepam / pharmacology |
| Chemical | |
Reg. No./Substance:
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0/Anticonvulsants; 0/GABA Modulators; 14176-49-9/Tiletamine; 31352-82-6/Zolazepam; EC 2.6.1.1/Aspartate Aminotransferases; EC 2.6.1.2/Alanine Transaminase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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