| Biochemical and molecular characterization of AtPAP12 and AtPAP26: the predominant purple acid phosphatase isozymes secreted by phosphate-starved Arabidopsis thaliana. | |
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MedLine Citation:
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PMID: 20545876 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Plant purple acid phosphatases (PAPs) belong to a large multigene family whose specific functions in Pi metabolism are poorly understood. Two PAP isozymes secreted by Pi-deficient (-Pi) Arabidopsis thaliana were purified from culture filtrates of -Pi suspension cells. They correspond to an AtPAP12 (At2g27190) homodimer and AtPAP26 (At5g34850) monomer composed of glycosylated 60 and 55 kDa subunit(s), respectively. Each PAP exhibited broad pH activity profiles centred at pH 5.6, and overlapping substrate specificities. Concanavalin-A chromatography resolved a pair of secreted AtPAP26 glycoforms. AtPAP26 is dual targeted during Pi stress because it is also the principal intracellular (vacuolar) PAP up-regulated by -Pi Arabidopsis. Differential glycosylation appears to influence the subcellular targeting and substrate selectivity of AtPAP26. The significant increase in secreted acid phosphatase activity of -Pi seedlings was correlated with the appearance of immunoreactive AtPAP12 and AtPAP26 polypeptides. Analysis of atpap12 and atpap26 T-DNA mutants verified that AtPAP12 and AtPAP26 account for most of the secreted acid phosphatase activity of -Pi wild-type seedlings. Semi-quantitative RT-PCR confirmed that transcriptional controls exert little influence on the up-regulation of AtPAP26 during Pi stress, whereas AtPAP12 transcripts correlate well with relative levels of secreted AtPAP12 polypeptides. We hypothesize that AtPAP12 and AtPAP26 facilitate Pi scavenging from soil-localized organophosphates during nutritional Pi deprivation. |
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Authors:
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Hue T Tran; Weiqiang Qian; Brenden A Hurley; Yi-Min She; Daowen Wang; William C Plaxton |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Plant, cell & environment Volume: 33 ISSN: 1365-3040 ISO Abbreviation: Plant Cell Environ. Publication Date: 2010 Nov |
Date Detail:
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Created Date: 2010-10-22 Completed Date: 2011-01-24 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9309004 Medline TA: Plant Cell Environ Country: United States |
Other Details:
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Languages: eng Pagination: 1789-803 Citation Subset: IM |
Copyright Information:
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© 2010 Blackwell Publishing Ltd. |
Affiliation:
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Department of Biology, Queen's University, Kingston, Ontario K7L3N6, Canada. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Acid Phosphatase
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genetics,
metabolism*,
secretion Amino Acid Sequence Arabidopsis / enzymology*, genetics Arabidopsis Proteins / genetics, metabolism*, secretion Computational Biology DNA, Bacterial / genetics Gene Expression Regulation, Enzymologic Gene Expression Regulation, Plant Glycosylation Hydrogen-Ion Concentration Molecular Sequence Data Mutation Phosphates / metabolism* RNA, Plant / genetics Seedling / enzymology* Sequence Alignment Substrate Specificity |
| Chemical | |
Reg. No./Substance:
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0/Arabidopsis Proteins; 0/DNA, Bacterial; 0/Phosphates; 0/RNA, Plant; 0/T-DNA; EC 3.1.3.2/Acid Phosphatase; EC 3.1.3.2/purple acid phosphatase 12, Arabidopsis |
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