| Biochemical mechanisms of methoxyfenozide resistance in the cotton leafworm Spodoptera littoralis. | |
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MedLine Citation:
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PMID: 19367570 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: Methoxyfenozide is a lepidopteran-specific insecticide that belongs to a new group of insecticides, the non-steroidal ecdysteroid agonists, also called moulting accelerating compounds (MACs). To investigate the risk of resistance and possible mechanisms conferring resistance to methoxyfenozide, the authors selected in the laboratory for a resistant strain of the cotton leafworm Spodoptera littoralis (Boisd.), which is a representative lepidopteran model and an important pest in cotton and vegetables worldwide, with a high risk for resistance development. RESULTS: After selection with methoxyfenozide during 13 generations, toxicity data showed that the selected strain developed fivefold resistance to methoxyfenozide in comparison with the susceptible strain. Measurement of the detoxification enzymes demonstrated that the monooxygenase (MO) activity was 2.1 times higher in the selected strain, whereas there was no change for esterases and glutathione-S-transferases. When the inhibitors piperonyl butoxide (PBO), S,S,S-tributyl phosphorotrithioate (DEF) and diethyl maleate were tested as synergists, the respective synergistic ratios were 0.97, 0.96 and 1.0 for the susceptible strain, and 2.2, 0.96 and 1.1 for the resistant strain. The significant synergistic effect by PBO concurs with the increased MO activity in the selected strain. CONCLUSION: Taken overall, the present study supports the importance of MO-mediated metabolism in resistance to methoxyfenozide, directing tactics to fight against resistance development for this novel group of insecticides. |
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Authors:
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Hadi Mosallanejad; Guy Smagghe |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Pest management science Volume: 65 ISSN: 1526-4998 ISO Abbreviation: Pest Manag. Sci. Publication Date: 2009 Jul |
Date Detail:
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Created Date: 2009-06-17 Completed Date: 2009-10-05 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 100898744 Medline TA: Pest Manag Sci Country: England |
Other Details:
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Languages: eng Pagination: 732-6 Citation Subset: IM |
Copyright Information:
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Copyright (c) 2009 Society of Chemical Industry. |
Affiliation:
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Laboratory of Agrozoology, Department of Crop Protection, Ghent University, Coupure Links 653, B-9000 Ghent, Belgium. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Esterases / genetics, metabolism Glutathione Transferase / genetics, metabolism Gossypium / parasitology Hydrazines / pharmacology* Insect Proteins / genetics, metabolism Insecticide Resistance* Insecticides / pharmacology* Juvenile Hormones / pharmacology* Mixed Function Oxygenases / genetics, metabolism Plant Diseases / parasitology Spodoptera / drug effects*, enzymology, physiology |
| Chemical | |
Reg. No./Substance:
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0/Hydrazines; 0/Insect Proteins; 0/Insecticides; 0/Juvenile Hormones; 0/methoxyfenozide; EC 1.-/Mixed Function Oxygenases; EC 2.5.1.18/Glutathione Transferase; EC 3.1.-/Esterases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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