Document Detail

Biochemical and immunohistochemical analyses of keratin expression in basal cell carcinoma.
MedLine Citation:
PMID:  9651824     Owner:  NLM     Status:  MEDLINE    
Recently we demonstrated that the keratin 17 (K17) content exceeded the K16 content in most follicular tumors, in comparison with non-follicular epithelial skin tumours by two-dimensional gel electrophoresis (2-DE), densitometry and immunohistochemistry. At present the origin of basal cell carcinoma (BCC) is unknown. So, based on the above results, we studied keratin expression in eight cases of BCC, in order to analyze tumor differentiation by both biochemical and immunohistochemical methods. Biochemically, using 2-DE and immunoblotting, stratified epithelial keratins K5/K14 and large amounts of K17 were present in all cases. Simple epithelial keratins K8 and K19 were expressed in all and half of the cases, respectively. However, hyperproliferative associated keratins (K6/K16) and keratinized keratins (K1/K10) were detected in only a few cases. Immunohistochemical studies using frozen sections with chain-specific antikeratin monoclonal antibodies against K1, K7, K8, K10, K14, K16, K17, K18 and K19 showed that BCC tumor cells reacted positively with antibodies against K8, K14, K17 and K19, but did not react, or were rarely positive with K1, K7, K10, K16 and K18 antibodies. Predominant expression of K17 and the frequent expression of K8 and K19, with little K6/K16 and K1/K10 expression are the characteristic features of BCC, suggesting that BCC is differentiated towards undifferentiated follicular epithelia, most probably hair bulge cells.
K Yoshikawa; Y Katagata; S Kondo
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of dermatological science     Volume:  17     ISSN:  0923-1811     ISO Abbreviation:  J. Dermatol. Sci.     Publication Date:  1998 May 
Date Detail:
Created Date:  1998-09-18     Completed Date:  1998-09-18     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9011485     Medline TA:  J Dermatol Sci     Country:  IRELAND    
Other Details:
Languages:  eng     Pagination:  15-23     Citation Subset:  IM    
Department of Dermatology, Yamagata University School of Medicine, Japan.
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MeSH Terms
Carcinoma, Basal Cell / metabolism*
Electrophoresis, Gel, Two-Dimensional
Keratins / metabolism*
Middle Aged
Skin Neoplasms / metabolism*
Reg. No./Substance:

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