Document Detail


Biochemical and immunochemical evidences for the presence of lipoxygenase in plant mitochondria.
MedLine Citation:
PMID:  15234996     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In this paper, both biochemical and immunochemical evidence for the presence of lipoxygenase (LOX) in plant mitochondria is presented. Highly purified pea (Pisum sativum L., cv. Alaska) mitochondria show LOX activity, evaluated as conjugated diene formation, oxygen consumption, and hydroperoxide formation. Both 9- and 13-hydroperoxy-octadecadienoic acids are produced by the oxidation of linoleic acid. LOX activity is particularly evident in swollen mitochondria; it is inhibited by nordihydroguaiaretic acid, a pea anti-LOX B antibody, and has two pH optima (6.0 and 7.5). A mitochondrial protein of approximately 97 kDa cross-reacts with a pea seed anti-LOX B antibody. This reaction is detectable in both soluble (matrix fraction) and membrane-bound (submitochondrial particles) proteins. Considering that pea mitochondria were extracted from actively growing stems that were differentiating tube elements, it is suggested that the presence of LOX in these organelles may be related to their degradation linked to xylem differentiation.
Authors:
Enrico Braidot; Elisa Petrussa; Stefano Micolini; Franco Tubaro; Angelo Vianello; Francesco Macrì
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2004-07-02
Journal Detail:
Title:  Journal of experimental botany     Volume:  55     ISSN:  0022-0957     ISO Abbreviation:  J. Exp. Bot.     Publication Date:  2004 Aug 
Date Detail:
Created Date:  2004-07-27     Completed Date:  2005-01-04     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9882906     Medline TA:  J Exp Bot     Country:  England    
Other Details:
Languages:  eng     Pagination:  1655-62     Citation Subset:  IM    
Affiliation:
Section of Plant Biology, Department of Biology and Agro-industrial Economics, University of Udine, via Cotonificio 108, I-33100 Udine, Italy.
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MeSH Terms
Descriptor/Qualifier:
Chromatography, High Pressure Liquid
Hydrogen-Ion Concentration
Isoenzymes
Lipoxygenase / analysis,  chemistry,  metabolism*
Microscopy, Electron, Transmission
Mitochondria / enzymology*
Peas / enzymology*
Time Factors
Chemical
Reg. No./Substance:
0/Isoenzymes; EC 1.13.11.12/Lipoxygenase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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