Document Detail

Biochemical and genetic evidence for a new class of emetine-resistant Chinese hamster cells with alterations in the protein biosynthetic machinery.
MedLine Citation:
PMID:  7444715     Owner:  NLM     Status:  MEDLINE    
We have isolated a number of emetine-resistant mutants from several different clones of CHO and CHL cells. Protein synthesis in extracts derived from each of the mutants is much more resistant to emetine than in the parental, emetine-sensitive cell lines, indicating the lesions affect the protein synthetic machinery directly. However, hybrid cell lines, derived from fusing either of two different emetine-resistant CHO mutants with either one of two different emetine-resistant CHL cells, are much more sensitive to growth inhibition by emetine than either parent. In addition, the incorporation of [3H] amino acids into protein in vivo and protein synthesis in vitro in extracts derived from these hybrids is much more sensitive to emetine inhibition than in either emetine-resistant parent. In contrast, no complementation was observed in hybrids derived from fusing two emetine-resistant CHO mutants or in hybrids derived from fusing two emetine-resistant CHL cell lines. These results indicate the CHO emetine-resistant mutants belong to one complementation group and the CHL emetine-resistant mutants belong to another. The genetic loci represented by these two complementation groups must both encode for gene products involved in protein synthesis.
J J Wasmuth; J M Hill; L S Vock
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Somatic cell genetics     Volume:  6     ISSN:  0098-0366     ISO Abbreviation:  Somatic Cell Genet.     Publication Date:  1980 Jul 
Date Detail:
Created Date:  1981-02-24     Completed Date:  1981-02-24     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7506054     Medline TA:  Somatic Cell Genet     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  495-516     Citation Subset:  IM    
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MeSH Terms
Cell Division
Cells, Cultured
Clone Cells / metabolism
Drug Resistance
Emetine / metabolism*,  pharmacology
Ethyl Methanesulfonate
Fibroblasts / metabolism
Genetic Complementation Test
Hybrid Cells / metabolism
Lung / cytology
Ovary / cytology
Protein Biosynthesis*
Ribosomal Proteins / genetics*
Reg. No./Substance:
0/Ribosomal Proteins; 483-18-1/Emetine; 62-50-0/Ethyl Methanesulfonate

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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