| Biochemical and functional characterization of an L-amino acid oxidase isolated from Bothrops pirajai snake venom. | |
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MedLine Citation:
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PMID: 16809041 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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In this work we describe the isolation of a new l-amino acid oxidase (LAAO) referred to as BpirLAAO-I from Bothrops pirajai snake venom, which was highly purified using a combination of molecular exclusion, affinity, and hydrophobic chromatography steps. BpirLAAO-I homodimeric acid glycoprotein (approximate Mr and pI of 130,000 and 4.9, respectively) displays high specificity toward hydrophobic/aromatic amino acids, while deglycosylation does not alter its enzymatic activity. The N-terminal LAAO sequence of its first 49 amino acids presented a high similarity between a amino acid sequence with other LAAOs from: Bothrops spp., Crotalus spp., Calloselasma rhodostoma, Agkistrodon spp., Trimeresurus spp., Pseudechis australis, Oxyuranus scutellatus, and Notechis scutatus. BpirLAAO-I induces time-dependent platelet aggregation, mouse paw edema, cytotoxic activity against Escherichia coli, Pseudomonas aeruginosa, Leishmania sp., and tumor cells, and also a typical fago (M13mp18) DNA fragmentation. Platelet aggregation, leishmanicidal and antitumoral activities were reduced by catalase. Thus, BpirLAAO-I is a multifunctional protein with promising biotechnological and medical applications. |
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Authors:
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Luiz Fernando M Izidoro; Marcela C Ribeiro; Guilherme R L Souza; Carolina D Sant'Ana; Amelia Hamaguchi; Maria I Homsi-Brandeburgo; Luiz R Goulart; Rene O Beleboni; Auro Nomizo; Suely V Sampaio; Andreimar M Soares; Veridiana M Rodrigues |
Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't Date: 2006-06-30 |
Journal Detail:
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Title: Bioorganic & medicinal chemistry Volume: 14 ISSN: 0968-0896 ISO Abbreviation: Bioorg. Med. Chem. Publication Date: 2006 Oct |
Date Detail:
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Created Date: 2006-09-05 Completed Date: 2006-11-24 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9413298 Medline TA: Bioorg Med Chem Country: England |
Other Details:
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Languages: eng Pagination: 7034-43 Citation Subset: IM |
Affiliation:
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Instituto de Genética e Bioquímica, Universidade Federal de Uberlândia, UFU, 38400-902 Uberlândia-MG, Brazil. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Animals Anti-Bacterial Agents* / chemistry, isolation & purification, pharmacology Bothrops* Catalysis Cell Line, Tumor Cell Proliferation / drug effects Cell Survival / drug effects Crotalid Venoms / chemistry* Edema / chemically induced Humans L-Amino Acid Oxidase* / chemistry, isolation & purification, pharmacology Mice Microbial Sensitivity Tests Molecular Sequence Data Platelet Aggregation / drug effects Time Factors |
| Chemical | |
Reg. No./Substance:
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0/Anti-Bacterial Agents; 0/Crotalid Venoms; EC 1.4.3.2/L-Amino Acid Oxidase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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