Document Detail


Biochemical and functional characterization of an L-amino acid oxidase isolated from Bothrops pirajai snake venom.
MedLine Citation:
PMID:  16809041     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In this work we describe the isolation of a new l-amino acid oxidase (LAAO) referred to as BpirLAAO-I from Bothrops pirajai snake venom, which was highly purified using a combination of molecular exclusion, affinity, and hydrophobic chromatography steps. BpirLAAO-I homodimeric acid glycoprotein (approximate Mr and pI of 130,000 and 4.9, respectively) displays high specificity toward hydrophobic/aromatic amino acids, while deglycosylation does not alter its enzymatic activity. The N-terminal LAAO sequence of its first 49 amino acids presented a high similarity between a amino acid sequence with other LAAOs from: Bothrops spp., Crotalus spp., Calloselasma rhodostoma, Agkistrodon spp., Trimeresurus spp., Pseudechis australis, Oxyuranus scutellatus, and Notechis scutatus. BpirLAAO-I induces time-dependent platelet aggregation, mouse paw edema, cytotoxic activity against Escherichia coli, Pseudomonas aeruginosa, Leishmania sp., and tumor cells, and also a typical fago (M13mp18) DNA fragmentation. Platelet aggregation, leishmanicidal and antitumoral activities were reduced by catalase. Thus, BpirLAAO-I is a multifunctional protein with promising biotechnological and medical applications.
Authors:
Luiz Fernando M Izidoro; Marcela C Ribeiro; Guilherme R L Souza; Carolina D Sant'Ana; Amelia Hamaguchi; Maria I Homsi-Brandeburgo; Luiz R Goulart; Rene O Beleboni; Auro Nomizo; Suely V Sampaio; Andreimar M Soares; Veridiana M Rodrigues
Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't     Date:  2006-06-30
Journal Detail:
Title:  Bioorganic & medicinal chemistry     Volume:  14     ISSN:  0968-0896     ISO Abbreviation:  Bioorg. Med. Chem.     Publication Date:  2006 Oct 
Date Detail:
Created Date:  2006-09-05     Completed Date:  2006-11-24     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9413298     Medline TA:  Bioorg Med Chem     Country:  England    
Other Details:
Languages:  eng     Pagination:  7034-43     Citation Subset:  IM    
Affiliation:
Instituto de Genética e Bioquímica, Universidade Federal de Uberlândia, UFU, 38400-902 Uberlândia-MG, Brazil.
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Animals
Anti-Bacterial Agents* / chemistry,  isolation & purification,  pharmacology
Bothrops*
Catalysis
Cell Line, Tumor
Cell Proliferation / drug effects
Cell Survival / drug effects
Crotalid Venoms / chemistry*
Edema / chemically induced
Humans
L-Amino Acid Oxidase* / chemistry,  isolation & purification,  pharmacology
Mice
Microbial Sensitivity Tests
Molecular Sequence Data
Platelet Aggregation / drug effects
Time Factors
Chemical
Reg. No./Substance:
0/Anti-Bacterial Agents; 0/Crotalid Venoms; EC 1.4.3.2/L-Amino Acid Oxidase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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