Document Detail

Biochemical factors affecting the fertility of cryopreserved human semen.
MedLine Citation:
PMID:  6677397     Owner:  NLM     Status:  MEDLINE    
The effects of dilution and cryopreservation on biochemical, fine structural and conventional semen parameters were studied and correlated with an index of fertility. Dilution of semen had major effects on the parameters studied. Freezing and thawing further reduced motility and the proportion of viable spermatozoa, and increased the proportion of spermatozoa with damaged membranes. The fertility index of donors was positively correlated with sperm concentration, seminal plasma protein concentration, damage to sperm head membranes and the proportion of spermatozoa with intact acrosomes prior to dilution or after freezing and thawing. Seminal plasma [Na+], [Ca2+], Ca2+/Mg2+-ratios and the change in [K+] were negatively correlated with fertility index. Multiple regression analysis revealed that [Na+] in seminal plasma, and sperm concentration in semen prior to dilution and freezing, contribute to 68% of the variation in fertility of cryopreserved semen.
M M Mahadevan; A O Trounson
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Clinical reproduction and fertility     Volume:  2     ISSN:  0725-556X     ISO Abbreviation:  Clin Reprod Fertil     Publication Date:  1983 Sep 
Date Detail:
Created Date:  1984-08-02     Completed Date:  1984-08-02     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8218028     Medline TA:  Clin Reprod Fertil     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  217-27     Citation Subset:  IM    
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MeSH Terms
Acid Phosphatase / analysis
Acrosome / analysis
Alkaline Phosphatase / analysis
Semen / analysis*
Semen Preservation / methods*
Sodium / analysis
Sperm Banks
Sperm Count
Sperm Head / analysis
Sperm Motility
Reg. No./Substance:
7440-23-5/Sodium; EC Phosphatase; EC Phosphatase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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